A nano composite film was fabricated by dispersing nano scale Ni(OH)(2) and carbon nanotubes (CNTs) into polyvinylidene fluoride (PVDF). The mophorlogy of the film was examined by scanning electron microscopy (SEM). The electrocatalytic oxidation of glucose in alkaline medium on the Ni(OH)(2)-CNT-PVDF (NCP) composite modified glass carbon electrode had been investigated. The stability of the composite is confirmed by cyclic voltammetry measurements in sodium hydroxide solution (0.50 M, scan rate 100 mV s(-1)). The NCP composite film maintains the electrocatalytic activity of the nano scale Ni(OH)(2) and is used to fabricate a nonenzymatic biosensor for electrochemical detection of glucose. Amperometric measurements were done with different concentrations of glucose. The NCP glucose sensor has good anti-interference performance toward maltose, fructose, urea, and ascorbic acid. It has wide concentration ranges to glucose. It shows a detection limit of 0.023 mM (S/N = 3) with a wide linear range from 0.25 to 39.26 mM, which is comparable with commercial glucose test strip. In real serum sample, it has a RSD of 2.5%. (C) 2014 The Electrochemical Society. All rights reserved.