As compared to the traditional solid Janus particles, the hollow Janus particles have inspired growing interests due to their diverse potential applications. Herein, the novel hollow Janus particles with elephant trunk-like and acorn-like shapes were prepared by seed emulsion polymerization. In contrast to traditional template methods, the hollow structure was obtained during the preparation by one-step swelling method. The shapes and internal structures of hollow Janus particles were confirmed, and the compositions were identified too. Some critical influences on the morphology control were investigated, that is, the surface modification, the amount of surfactant, and cross-linking agent concentrations. It was inferred that the balance of hydrophilicity and hydrophobicity and the effective phase separation were important for preparing the hollow Janus particles with tailored shapes. Finally, amphiphilic properties of hollow Janus particles were demonstrated by emulsifying oil-water mixture.