Chitosan is a good biocompatible polysaccharide obtained from deacetylation of chitin and has been widely used in biomedical fields. In the present study, chitosan immobilization to a biodegradable poly(lactic-co-glycolic acid) (PLGA) for hepatocyte seeding was investigated. The immobilization agent was N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) in N-hydroxysuccinimide (NHS) and 2-morpholinoethane sulfonic acid MES buffer. Scanning electron microscopy (SEM) observation displayed little difference in surface morphology between the chitosan-modified film and the control. But water contact angles decreased with an increase of chitosan concentration in cross-linking solvent and the reaction time. Electron spectroscopy for chemical analysis (ESCA) showed an obvious additional peak in the chitosan-modified PLGA film due to nitrogen atoms, based on the considerable amount of amino groups in chitosan molecules. Hepatocyte culture indicated that chitosan could improve the biocompatibility of PLGA scaffold.