The plastic deformation behavior of Ni3Ti single crystals with the D0(24) structure was investigated in this study. {1(1) over bar 00}<11(2) over bar 0 > prism slip and (0001)<2(11) over bar 0 > basal slip were operative in Ni3Ti. Anomalous strengthening of yield stress was observed for both slip systems. Especially, a remarkable yield stress anomaly (YSA) was found for the (0001) basal slip system over a wide temperature range between 300 and 800degreesC. The YSA was attributed to the Kear-Wilsdorf locking of 1/3<2(11) over bar 0 > screw dislocations from the (0001) slip plane onto the {01(1) over bar 0} planes.