Biological systems operate at a multi-component and hierarchical scale that requires the participation of specific cues (i.e., cells, proteins) orchestrated in a precise spatial and temporal fashion. Our objective is to establish a robust and multifunctional material construct that provides multiple growth and developmental signals to enhance cellular function and to assist in the re-establishment of normal tissue architecture. We developed an in situ photopolymerized interpenetrating network (IPN) system that consists of (i) gelatin modified with polyanions and/or polyethyleneglycols (PEG) and (ii) PEG-diacrylate at various ratios. Immobilized and soluble factors were incorporated within the IPN to provide various biological signals in modulating cellular behavior. The effect of formulation on IPN chemicophysical properties, drug release kinetics, in vitro cell interaction thereof, and in vivo host response were quantified.