Significant plastic deformation of generally brittle materials can be realized by indentation. Therefore indentation techniques are suitable for investigation of the plastic deformation of low-ductile materials. In this work the deformation of ZrTiCuNiBe metallic glass at room temperature was studied via hardness measurement and instrumented nanoindentation. The stress-strain curve for the alloy was obtained using 9 pyramidal indenters with various angles of sharpening. The analysis of this curve allows a conclusion that the room-temperature deformation of metallic glasses occurs in two stages. The homogeneous deformation of the material takes place in the first stage. When the flow stress increases to some critical value due to strain hardening, the second stage begins, and the inhomogeneous (localized) plastic flow occurs. The results of the alloy investigation by cyclic nanoindentation confirm the aforementioned conclusion and show that the stress-overshoot effect exists in metallic glasses at room temperature.