The influence of microstructure concerning sigma phase on hydrogen behavior was investigated by means of internal friction analysis. After hydrogen charging, a sharp significant internal friction peak by hydrogen in austenite of as-received specimen was observed at 245K for a frequency of 1.5Hz. However, the peak height in the specimen precipitated significant sigma phase was substantially lower than in as-received specimen since hydrogen in austenite have a concentration lower by sigma phase precipitation. In addition, the broadening and scattering of the internal friction peak was clearly identified by interaction between hydrogen and sigma phase. It means that the two peaks associated with hydrogen in the both sigma phase and austenite were considered to be overlapped. Consequently, it was clearly confirmed that hydrogen entered in the sigma phase lattice and hydrogen was also trapped at sigma/austenite interfaces.