감초 플라보노이드 Liquiritin 및 Liquiritigenin을 담지한 피부전달체인 셀룰로오스 다공성 하이드로젤 제형 개발

김수지; 권순식; 유은령; 박수남; Su Ji Kim; Soon Sik Kwon; Eun Ryeong Yu; Soo Nam Park

Polymer(Korea), Vol.38, No.5, 676-681, September, 2014

Development of Porous Cellulose Hydrogel for Enhanced Transdermal Delivery of Liquiritin and Liquiritigenin as Licorice Flavonoids

김수지, 권순식, 유은령, 박수남 ^†

서울과학기술대학교 에너지바이오대학 정밀화학과, 화장품종합기술연구소

Su Ji Kim, Soon Sik Kwon, Eun Ryeong Yu, and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D Center, College of Energy and Biotechnology, Soeul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 137-743, Korea

E-mail:

초록

한약재로 널리 사용되는 감초는 항산화/항염증 등의 작용을 나타내는 배당체 liquiritin 및 그 아글리콘인 liquiritigenin과 같은 플라보노이드를 함유하고 있다. 본 연구에서는 피부 전달체로 이들 플라보노이드를 함유하는 하이드로젤을 제조하고 그 특성과 피부 투과능을 조사하였다. 셀룰로오스와 NaOH/urea(1~10%) 용액 및 가교제로서 에피클로로하이드린을 사용하여 셀룰로오스 다공성 하이드로젤을 제조하였다. Liquiritin 및 liquiritigenin 담지를 위한 최적의 하이드로젤은 셀룰로오스 용해를 위해 사용된 1~10%의 우레아 농도 중 6% 우레아 용액에서 제조된 하이드로젤이 동적 점탄성 및 수분 흡수능이 가장 우수한 것으로 나타났다. SEM으로 관찰한 결과, 제조된 하이드로젤의 단면은 다공성을 나타내었다. Franz diffusion cell을 이용한 in vitro 피부투과 실험 결과, 감초 플라보노이드 함유하이드로젤은 대조군보다 더 높은 피부 투과능을 나타내었다. 이상의 결과들은 셀룰로오스 다공성 하이드로젤이 감초 플라보노이드의 경피 전달에 있어 효율적인 피부 전달체로 이용 가능성이 있음을 시사한다.

Licorice, widely used as a herbal medicine, has flavonoids such as liquiritin and its aglycone, liquiritigenin that show anti-oxidant and anti-inflammatory properties. Licorice flavonoid-loaded cellulose hydrogels were prepared as carriers for skin drug delivery, and their properties were investigated. The porous cellulose hydrogel was made by reacting cellulose with epichlorohydrin as a cross-linking agent in NaOH/urea(1~10%) solutions. Through studies on the rheological properties and water uptake of the hydrogel, a NaOH/urea(6%) solution was established as being optimum for the synthesis of the cellulose hydrogel containing liquiritin and liquiritigenin. Scanning electron microscopy (SEM) observations of a cross-section of the prepared hydrogel indicated its porosity. In particular, in skin permeation experiments using a Franz diffusion cell, hydrogel containing the licorice flavonoids showed remarkable transdermal permeation compared to the control group. These results indicate that porous cellulose hydrogel is a potential drug delivery system to enhance the skin permeation of licorice flavonoids.

Keywords:liquiritin;liquiritigenin;cellulose;hydrogel;drug delivery system.

[References]

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Kim MY, Jung B, Park JH, Biomaterials, 33, 668 (2012)
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Zhang P, Zhang L, Cheng S, Carbohyd. Res., 327, 431 (2007)
Lee YH, Fan LT, Biotechnol. Bioeng., 25, 2707 (1983)
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Packer L, New Compr. Biochem., 28, 239 (1994)
Francesco B, Maria L, Lucia M, Claudio P, Antonio T, Domenico T, Francesco C, Antonella S, Int. J. Pharm., 145, 87 (1996)
Wang S, Gue M, Cong J, Li S, J. Chromatogr. A, 1282, 167 (2013)
Kim HJ, Bae JY, Jang HN, Park SN, Korean J. Microbiol. Biotechno., 41, 358 (2013)
Thiele JJ, Schroeter C, Hsieh SN, Podda M, Packer L, Thiele J, Elsner P, Curr. Probl. Dermatol., 29, 26 (2001)
Lim MS, Han SB, Kwon SS, Park MA, Park SN, J. Soc. Cosmet. Scientists Korea, 38, 147 (2012)
Sylvie VS, Bonte F, J. Cosmet. Dermatol., 6, 75 (2007)
Bouwstra JA, Maria P, Biochimica et Biophysica Acta, 1758, 2080 (2006)
Lee MH, Kim SJ, Park SN, Polym.(Korea), 37(3), 347 (2013)
Park JH, Master Degree, Sungkyunkwan University, Korea (2008)
Pasqui D, Cagna MD, Barbucci R, Polymers, 4, 1517 (2012)
Lee HJ, Master Degree, Seoul National University of Science and Technology, Korea (2012)
Heather AEB, Curr. Drug Deliv., 2, 23 (2005)

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[3] Kim MY, Jung B, Park JH, Biomaterials, 33, 668 (2012)

[4] Lee MS, Hong SK, Kim JC, Polym. Sci. Technol., 23(1), 13 (2012)

[5] Ciolacu D, Oprea AM, Anghel N, Cazacu G, Cazacu M, Mater. Sci. Eng. C, 32, 452 (2012)

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[10] Park SN, J. Soc. Cosmet. Scientists Korea, 23, 75 (1997)

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