The microspheres were prepared by emulsification method using glutaraldehyde as crosslinker. The effects of variables as temperature, concentration of Isabgol husk and glutaraldehyde, and nature of dispersing media were analyzed on microsphere-derived properties such as size distribution, surface morphology, and swelling kinetics. The nature of crosslinking and interaction of polysaccharides structure with glutaraldehyde were assessed by thermal analysis and infrared spectroscopy. The spherical and free flowing microspheres were obtained and a significant effect of process variables was observed on characteristics of formulations. The microspheres revealed their potential in the development of sustained release drug carriers for water soluble/insoluble drugs.