Ethanolysis of squalene free shark liver oil was carried out to obtain a product mixture comprised mainly of fatty acid ethyl esters and non-esterified alkylglycerols. Purification of non-esterified alkylglycerols from this product mixture via supercritical fluid extraction and short-path distillation has been compared. The effect of temperature and mass flow in a sequential distillation strategy was evaluated. In addition, combination of supercritical fluid extraction and short-path distillation to obtain a product highly enriched in alkylglycerols readily for human consumption was also studied. (C) 2011 Elsevier B.V. All rights reserved.