Gelatin Modification with Photocuring Thymine Derivative and its Application for Hemostatic Aid

Dong June Chung; Takehisa Matsuda

Journal of Industrial and Engineering Chemistry, Vol.4, No.4, 340-344, December, 1998

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Gelatin Modification with Photocuring Thymine Derivative and its Application for Hemostatic Aid

Dong June Chung^†, and Takehisa Matsuda¹

Department of Polymer Science and Engineering, SungKyunKwan University, Suwon 440-746, Korea
¹Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka 565, Japan

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Gelatin was derivatized with [1-(2-carboxyethyl)thymine)] via coupling reaction with lysinyl amine groups of gelatin in the presence of a water-soluble carbodiimide. Ultraviolet (UV) irradiation on photosensitive thyminated gelatin films resulted in the formation of crosslinked gelatin gels. Degrees of swelling and photocuring depended on the thymine content of gelatin and irradiation time. The crosslinking rate and physical properties of photocured gelatin are controlled by the degree of derivatization and photo-irradiation time. Photocuring of a viscous thyminated gelatin solution coated on a mechanically injured liver surface of rat was demonstrated for possible use as a sealant or hemostatic aid for bleeding during laparoscopic procedures.

Keywords:Gelatin;Photocuring;Modified Thymine;Hemostatic Aid

[References]

Cheung DT, Nimni ME, Connect. Tissue Res., 10, 201 (1982)
Barker H, Oliver R, Grant R, Stephan L, Biochim. Biophys. Acta, 632, 589 (1980)
Weadock K, Olson RM, Silve FH, Artif. Org., 11, 293 (1983)
Cote MF, Doillon CT, Biomaterials, 13, 612 (1992)
Nakayama Y, Matsuda T, J. Polym. Sci. A: Polym. Chem., 30, 2451 (1992)
Matsuda T, Moghaddam MJ, Miwa H, Sakurai K, Iida F, ASAIO J., 38, 243 (1992)
Moghaddam MJ, Matsuda T, Trans. Am. Soc. Artif. Int. Org., 37, 1437 (1991)
Matsuda T, Miwa H, Moghaddam MJ, Mathew J, Iida F, ASAIO J., 39, 327 (1993)
Overberger CG, Inaki Y, J. Polym. Sci. A: Polym. Chem., 17, 1739 (1979)
Habeeb AFSA, Anal. Biochem., 14, 328 (1966)
Kito H, Nakajima N, Matsuda T, ASAIO J., 39, 506 (1993)

[Referenced By]

[Related Articles]

[1] Cheung DT, Nimni ME, Connect. Tissue Res., 10, 201 (1982)

[2] Barker H, Oliver R, Grant R, Stephan L, Biochim. Biophys. Acta, 632, 589 (1980)

[3] Weadock K, Olson RM, Silve FH, Artif. Org., 11, 293 (1983)

[4] Cote MF, Doillon CT, Biomaterials, 13, 612 (1992)

[5] Nakayama Y, Matsuda T, J. Polym. Sci. A: Polym. Chem., 30, 2451 (1992)

[6] Matsuda T, Moghaddam MJ, Miwa H, Sakurai K, Iida F, ASAIO J., 38, 243 (1992)

[7] Moghaddam MJ, Matsuda T, Trans. Am. Soc. Artif. Int. Org., 37, 1437 (1991)

[8] Matsuda T, Miwa H, Moghaddam MJ, Mathew J, Iida F, ASAIO J., 39, 327 (1993)

[9] Overberger CG, Inaki Y, J. Polym. Sci. A: Polym. Chem., 17, 1739 (1979)

[10] Habeeb AFSA, Anal. Biochem., 14, 328 (1966)

[11] Kito H, Nakajima N, Matsuda T, ASAIO J., 39, 506 (1993)