In this work for the first time Ion-Jelly (R) membranes were developed and characterized. Ion-Jelly (R) is a new green and sustainable gel material, obtained by addition of an ionic liquid to gelatine. Three different ionic liquids ([EMIM][DCA], [BMIM][DCA] and [BMPyr][DCA]), were used to study the difference in the membrane properties introduced by changes in the ionic liquid cation. The physical properties of the membranes were characterized by traction, contact angles, and SEM. These membranes were further tested in separation processes using supercritical carbon dioxide for the fractionation of a model mixture made of oleic acid and squalene and for the separation of biodiesel products and reagents. The permeability to pure gases (H-2, N-2, O-2, CO2 and CH4) was also determined and ideal selectivities calculated. (C) 2012 Elsevier B.V. All rights reserved.