While the use of nanostructured metal oxides such as nanotitania, nanozirconia, nanoalumina, and mesoporous alumina, as column matrices constitutes a successful paradigm in the development of chromatographic Mo-99/Tc-99m generators using (n,gamma)Mo-99, a comparative assessment of their properties is essential not only to determine the merits and bottlenecks of each sorbent but also to evaluate their usefulness commensurate with the specific activity of (n,gamma)Mo-99 obtained from different sources. Characteristics which were compared included the sorption capacity, zeta potential, shelf-life of the generator, radioactive concentration and purity of Tc-99m for radiopharmaceutical applications, and recovery of decayed Mo from spent generators. Mesoporous alumina was identified as the most suitable sorbent for ensuring sustainable production of clinical grade Mo-99/Tc-99m generators using low specific activity Mo-99.