A theory of nonlinear inverse chromatography for arbitrary adsorption isotherms has been developed. Methods of investigating adsorption isotherms and heat of adsorption based on the analysis of chromatograms for different temperatures are suggested. Simple formulae for calculating of adsorption isotherms and heat of adsorption are derived. The adsorption isotherm and heat of adsorption of pyridine on particular poly(imide)siloxane have been investigated by inverse chromatography.