Three-dimensional ordered macroporous carbon (3DOM-C) was synthesized through polystyrene (PS) colloidal crystal templates using carbonized glucose solutions as a carbon source and sulfuric acid as a dehydrating agent. The obtained samples were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and nitrogen-sorption isotherm measurement. SEM results showed that the layers of the PS templates were hexagonal close-packed with a large surface area, and the diameter of the PS microspheres was 300-320 nm. SEM and TEM results demonstrated that the 3DOM-C displayed a unique structure of a well-developed 3D-interconnected ordered macroporous framework with a pore diameter of 260280 nm. The photovoltaic current voltage curves showed that the dye sensitized solar cells (DSSC) fabricated by the 3DOM-C as a counter electrode exhibited an energy conversion efficiency (eta = 2.91%), a short circuit current (J(sc) = 9.51 mA/cm(2)), an open circuit voltage (V-oc = 0.59 V) with a fill factor (FF = 52%), which were much closed to those of Pt counter electrode-based DSSC (3.59%, 10.05 mA/cm(2), 0.59 V, 58%) and better than those of activated carbon-based one. Moreover, the electrochemical impedance spectroscopy analysis demonstrated a low charge-transfer resistance (R-CT) for 3DOM-C (3.48 Omega cm(2)), which was close to that of Pt (1.54 Omega cm(2)), and much lower than that of activated carbon (15.05 Omega cm(2)). Finally, the 3DOM-C showed a comparable efficiency compared to a conventional Pt counter electrode. (C) 2013 Elsevier B.V. All rights reserved.