Direct ethanol production from dextran industrial waste water by Zymomonas mobilis

Ming-xiong He; Han Qin; Xiao-bo Yin; Zhi-yong Ruan; Fu-rong Tan; Bo Wu; Zong-xia Shui; Li-chun Dai; Qi-chun Hu

Korean Journal of Chemical Engineering, Vol.31, No.11, 2003-2007, November, 2014

DOI10.1007/s11814-014-0108-1 Export Citation

Direct ethanol production from dextran industrial waste water by Zymomonas mobilis

Ming-xiong He^{1, 2, †}, Han Qin¹, Xiao-bo Yin², Zhi-yong Ruan³, Fu-rong Tan¹, Bo Wu¹, Zong-xia Shui¹, Li-chun Dai¹, and Qi-chun Hu^{1, 2}

¹Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, Chengdu 610041, China
²Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Chengdu 610041, P. R. China
³Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China

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The direct production of ethanol from dextran industrial waste water was investigated by using Zymomonas mobilis via batch and semi-continuous fermentation mode. In batch fermentation, pretreated waste water (unsterilized and sterilized), pH value (3.8 and 6.0), and Mg2+ (with and without) was compared with OD600, sugar and ethanol concentration. After 24 h fermentation, sugar in the dextran waste water was almost exhausted, and the amount of ethanol accumulated reached 24.33-29.92 g/l, which is nearly 99% of the theoretical yield of ethanol. Kinetic parameters of Z. mobilis in batch fermentation were also investigated. The raw dextran waste water was also used in semi-continuous fermentation. After 48 h fermentation, the production of ethanol was 28.65 g/l. These results indicated that dextran waste water may be used as a candidate substrate and Z. mobilis could convert the raw material into ethanol directly.

Keywords:Ethanol;Dextran Industrial Waste Water;Zymomonas mobilis;Batch Fermentation;Kinetic Parameters;Semicontinuous Fermentation

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[2] Govumoni SP, Koti S, Kothagouni SY, Linga VR, Carbohyd. Polym., 91, 646 (2013)

[3] Galbe M, Zacchi G, Biomass Bioenerg., 46, 70 (2012)

[4] Van Dyk JS, Pletschke BI, Biotechnol. Adv., 30, 1458 (2012)

[5] Klein-Marcuschamer D, Oleskowicz-Popiel P, Simmons BA, Blanch HW, Biotechnol. Bioeng., 109(4), 1083 (2012)

[6] Seo JS, Chong H, Park HS, Yoon KO, Jung C, Kim JJ, et al., Nat. Biotechnol., 23, 63 (2005)

[7] Matsushika A, Inoue H, Kodaki T, Sawayama S, Appl. Microbiol. Biotechnol., 84(1), 37 (2009)

[8] Jang YS, Park JM, Choi S, Choi YJ, Seung DY, Ch JH, et al., Biotechnol. Adv., 30, 989 (2012)

[9] Kambam PKR, Henson MA, Biofuels., 1, 729 (2010)

[10] Zhang W, Geng A, Biotechnol. Biofuels., 5, 46 (2012)

[11] Zhang M, Eddy C, Deanda K, Finkestein M, Picataggio S, Science, 267(5195), 240 (1995)

[12] Deanda K, Zhang M, Eddy C, Picataggio S, Appl. Env. Microbiol., 62, 4465 (1996)

[13] Linger JG, Adney WS, Darzins A, Appl. Environ. Microbiol., 76, 6360 (2010)

[14] Jarboe LR, Grabar TB, Yomano LP, Shanmugan KT, Ingram LO, Biofuels., 237 (2007)

[15] Zhou S, Yomano LP, Shanmugam KT, Ingram LO, Biotechnol. Lett., 27(23-24), 1891 (2005)

[16] Kim Y, Ingram LO, Shanmugam KT, Appl. Environ. Microbiol., 73, 1766 (2007)

[17] Wang Y, Manow R, Finan C, Wang J, Garza E, Zhou S, J. Ind. Microbiol. Biotechnol., 38, 1371 (2010)

[18] Causey TB, Zhou S, Shanmugam KT, Ingram LO, Proc. Natl. Acad. Sci. USA, 100, 825 (2003)

[19] Dunlop M, Biotechnol. Biofuels., 4, 32 (2011)

[20] Alper H, Moxley J, Nevoigt E, Fink GR, Stephanopoulos G, Science, 314, 1565 (2006)

[21] Pienkos P, Zhang M, Cellulose., 16, 743 (2009)

[22] He MX, Wu B, Shui ZX, Hu QC, Wang WG, Tan FR, Tang XY, Zhu QL, Pan K, Li Q, Su XH, Appl. Microbiol. Biotechnol., 95(1), 189 (2012)

[23] He MX, Wu B, Shui ZX, Hu QC, Wang WG, Tan FR, et al., Biotechnol. Biofuels., 5, 75 (2012)

[24] Ross AB, Jones JM, Kubacki ML, Bridgeman T, Bioresour. Technol., 99(14), 6494 (2008)

[25] Park JH, Hong JY, Jang HC, Oh SG, Kim SH, Yoon JJ, Kim YJ, Bioresour. Technol., 108, 83 (2012)

[26] Tan IS, Lam MK, Lee KT, Carbohyd. Polym., 94, 561 (2013)

[27] Brown DE, McAvoy A, J. Chem. Technol. Biotechnol., 48, 405 (1990)

[28] Goodman AE, Rogers PL, Scotnicki M, Appl. Env. Microbiol., 44, 496 (1982)

[29] He MX, Wu B, Shui ZX, Hu QC, Wang WG, Tan FR, Tang XY, Zhu QL, Pan K, Li Q, Su XH, Appl. Microbiol. Biotechnol., 95(1), 189 (2012)

[30] He MX, Wu B, Shui ZX, Hu QC, Wang WG, Tan FR, Tang XY, Zhu QL, Pan K, Li Q, Su XH, Biotechnol. Biofuel., 5, 75 (2012)

[31] Kim IS, Barrow KD, Rogers PL, Appl. Env. Microbiol., 66, 186 (2000)

[32] Jeon YI, Svenson CJ, Rogers PL, FEMS Microbiol. Lett., 244, 85 (2005)

[33] Jeffries TW, Nat. Biotechnol., 23, 40 (2005)

[34] Swings J, Deley J, Bacterial. Rev., 41, 1 (1977)