Immobilization of glucose oxidase attached to new nanospheres including azomethine

Nurbanu Ozdem; Elvan Hasanoglu Ozkan; Nursen Sarı; Fatma Arslan; Hayrettin Tomturk

Macromolecular Research, Vol.22, No.12, 1282-1287, December, 2014

DOI10.1007/s13233-014-2178-7 Export Citation

Immobilization of glucose oxidase attached to new nanospheres including azomethine

Nurbanu Ozdem, Elvan Hasanoglu Ozkan, Nursen Sarı^†, Fatma Arslan, and Hayrettin Tomturk

Department of Chemistry, Faculty of Science, Gazi University, 06500, Teknikokullar, Ankara, Turkiye, Turkey

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New nanosphere supports were prepared and characterized for the immobilization of glucose oxidase from Aspergillus niger. Nanoparticles ((APS-tio), (APS-tioCH3), and (APS-tioCl)) modified (aminomethyl)polystyrene (APS) with some tyophenealdehyde derivatives were synthesized by means of condensation and we investigated the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on them. Modified polystyrene was characterized by IR spectra, gel permeation chromatography and scanning electron microscopy. Immobilized GOx on (APS-tioCH3) showed a optimum pH, whereas immobilized GOx on (APS-tio) and (APS-tioCl) performed the optimum condition at two pHs.

Keywords:biocatalysis;crystal;glucose oxidase;nanospheres

[References]

Aynaci E, Sari N, Tumturk H, Artif. Cells Blood Substit. Immobil. Biotechnol., 39, 259 (2011)
Erduran V, Tumturk H, Aynaci E, Sari N, J. Macromol. Sci. A, 50, 871 (2013)
David AE, Wang NS, Yang VC, Yang AJ, J. Biotechnol., 125, 395 (2006)
Bozkir E, Sari N, Ogutcu H, J. Inorg. Organomet. Polym., 22, 1146 (2012)
Hanf F, Koehler P, J. Sci. Food Agric., 86, 1699 (2006)
Yu J, Liu S, Ju H, Biosens. Bioelctron., 19, 401 (2003)
Arslan H, Ozdemir M, Zengin H, Int. J. Electrochem. Sci., 7, 10205 (2012)
Ge J, Lu D, Liu Z, Lui Z, Biochem. Eng. J., 44, 53 (2009)
Moyo M, Okonkwo JO, Agyei MN, Sensors (Basel), 12, 923 (2012)
Aksoy S, Tumturk H, Hasirci N, J. Biotechnol., 60, 37 (1998)
Guisan JM, Immobilization of Enzymes and Cells, Methods in Biotechnology, Humana Press Inc., Totowa, 2, 14 (2006)
Huang J, Zhao R, Wang H, Zhao WQ, Ding LY, Biotechnol. Lett., 32(6), 817 (2010)
Guo CX, Li CM, Phys. Chem. Chem. Phys., 12, 12153 (2010)
Arslan F, Ustabasi S, Arslan H, Sensors, 11, 8152 (2011)
Cil M, Buyukbayram AE, Kiralp S, Toppare L, Yagci Y, Int. J. Bio. Macromol., 41, 49 (2007)
Xu XH, Ren GL, Cheng J, Liu Q, Li DG, Chen Q, J. Mater. Sci., 41(10), 3147 (2006)
Trinder P, Ann. Clin. Biochem., 6, 24 (1969)
Sari N, Ozcan S, Chinese J. Polym. Sci., 27, 675 (2009)
Sari N, Yuzuak N, J. Inorg. Organomet. Polym. Mater., 16, 259 (2006)
Silverstein RM, Webster FX, Spectrometric Identification of Organic Compounds, 6th ed., John Wiley and Sons, Inc., New York, p 86. (1997)
Sari N, Antepli E, Nartop D, Yetim NK, Int. J. Mol. Sci., 13, 11870 (2012)
Wang X, Zhu KX, Zhou HM, Int. J. Mol. Sci., 12(5), 3042 (2011)
Colak O, Yasar A, Cete S, Arslan F, Artif. Cells Blood Substit. Immobil. Biotechnol., 40, 354 (2012)
Gorgulu M, Cete S, Arslan H, Yasar A, Artif. Cells Blood Substit. Immobil. Biotechnol., 41, 327 (2013)

[1] Aynaci E, Sari N, Tumturk H, Artif. Cells Blood Substit. Immobil. Biotechnol., 39, 259 (2011)

[2] Erduran V, Tumturk H, Aynaci E, Sari N, J. Macromol. Sci. A, 50, 871 (2013)

[3] David AE, Wang NS, Yang VC, Yang AJ, J. Biotechnol., 125, 395 (2006)

[4] Bozkir E, Sari N, Ogutcu H, J. Inorg. Organomet. Polym., 22, 1146 (2012)

[5] Hanf F, Koehler P, J. Sci. Food Agric., 86, 1699 (2006)

[6] Yu J, Liu S, Ju H, Biosens. Bioelctron., 19, 401 (2003)

[7] Arslan H, Ozdemir M, Zengin H, Int. J. Electrochem. Sci., 7, 10205 (2012)

[8] Ge J, Lu D, Liu Z, Lui Z, Biochem. Eng. J., 44, 53 (2009)

[9] Moyo M, Okonkwo JO, Agyei MN, Sensors (Basel), 12, 923 (2012)

[10] Aksoy S, Tumturk H, Hasirci N, J. Biotechnol., 60, 37 (1998)

[11] Guisan JM, Immobilization of Enzymes and Cells, Methods in Biotechnology, Humana Press Inc., Totowa, 2, 14 (2006)

[12] Huang J, Zhao R, Wang H, Zhao WQ, Ding LY, Biotechnol. Lett., 32(6), 817 (2010)

[13] Guo CX, Li CM, Phys. Chem. Chem. Phys., 12, 12153 (2010)

[14] Arslan F, Ustabasi S, Arslan H, Sensors, 11, 8152 (2011)

[15] Cil M, Buyukbayram AE, Kiralp S, Toppare L, Yagci Y, Int. J. Bio. Macromol., 41, 49 (2007)

[16] Xu XH, Ren GL, Cheng J, Liu Q, Li DG, Chen Q, J. Mater. Sci., 41(10), 3147 (2006)

[17] Trinder P, Ann. Clin. Biochem., 6, 24 (1969)

[18] Sari N, Ozcan S, Chinese J. Polym. Sci., 27, 675 (2009)

[19] Sari N, Yuzuak N, J. Inorg. Organomet. Polym. Mater., 16, 259 (2006)

[20] Silverstein RM, Webster FX, Spectrometric Identification of Organic Compounds, 6th ed., John Wiley and Sons, Inc., New York, p 86. (1997)

[21] Sari N, Antepli E, Nartop D, Yetim NK, Int. J. Mol. Sci., 13, 11870 (2012)

[22] Wang X, Zhu KX, Zhou HM, Int. J. Mol. Sci., 12(5), 3042 (2011)

[23] Colak O, Yasar A, Cete S, Arslan F, Artif. Cells Blood Substit. Immobil. Biotechnol., 40, 354 (2012)

[24] Gorgulu M, Cete S, Arslan H, Yasar A, Artif. Cells Blood Substit. Immobil. Biotechnol., 41, 327 (2013)