화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.22, No.12, 1320-1329, December, 2014
DOI10.1007/s13233-014-2183-x  Export Citation
Dual growth factor-loaded core-shell polymer microcapsules can promote osteogenesis and angiogenesis
Ramesh Subbiah1, 2, Ping Du1, 2, Mintai Peter Hwang2, In Gul Kim2, Se Young Van1, 2, Yong Kwan Noh2, 3, Hansoo Park4, and Kwideok Park1, 2, †
  • 1Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejon, 305-350, Korea
  • 2Center for Biomaterials, Korea Institute of Science and Technology (KIST), Seoul, 136-130, Korea
  • 3Department of Biomedical Engineering, School of Medicine, Kyunghee University, Korea
  • 4School of Integrative Engineering, Chung-Ang University, Seoul, 156-071, Korea
E-mail:
Growth factors (GFs) are very critical in stem cell differentiation and tissue regeneration. Therefore GF delivery carriers have been a major subject in tissue engineering research. In this study, we prepare and optimize core-shell microcapsules (C-S MCs) for dual GF delivery. The C-S MCs, composed of an alginate shell and poly(lactic-co-glycolic) acid (PLGA) core, are fabricated using an electrodropping method via custom-made coaxial needles. They are 198±38 μm in diameter with an average core size of 90±13 μm, and they are fabricated using an alginate concentration of 1% (w/v), an electrical voltage of 11 kV, and an inner syringe flow rate of 50 μL/min. Using this platform, dual GFs, bone morphogenetic protein (BMP-2) and vascular endothelial growth factor (VEGF) are encapsulated in the alginate shell and PLGA core, respectively. In vitro release tests of dual GF-loaded C-S MCs reveal early release of BMP-2, followed by VEGF on a temporal release profile of 28 days. In vitro study of the dual GF-loaded MCs demonstrates their osteogenic activity with preosteoblasts; osteogenic markers (osteocalcin and type I collagen) are upregulated and both calcium content and alkaline phosphatase (ALP) activity also increased. In addition, C-S MCs combined with collagen and preosteoblasts were subcutaneously transplanted to the dorsal region of nude mice for 3 weeks. Analysis of the retrieved constructs exhibits that both osteogenesis and angiogenesis were more active in the group containing dual GF-loaded MCs, along with deep penetration of blood vessels inside the construct, compared to blank MCs or single GF (BMP-2)-loaded MCs. This study proposes a dual GF delivery carrier using C-S MCs and demonstrates the feasibility of C-S MCs in the induction of osteogenesis and angiogenesis.
Keywords:core-shell microcapsules;dual growth factor delivery;electrodropping;osteogenesis;angiogenesis
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