Effect of bed configuration of immobilized biocatalysts on penicillin G hydrolysis efficiency

Anca-Irina Galaction; Alexandra Cristina Blaga; Ramona Mihaela Matran; Dan Caşcaval

Korean Journal of Chemical Engineering, Vol.32, No.2, 216-221, February, 2015

DOI10.1007/s11814-014-0231-z Export Citation

Effect of bed configuration of immobilized biocatalysts on penicillin G hydrolysis efficiency

Anca-Irina Galaction, Alexandra Cristina Blaga¹, Ramona Mihaela Matran¹, and Dan Caşcaval^†

Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy of Iasi, M. Kogalniceanu 9-13, Iasi 700454, Romania
¹Faculty of Chemical Engineering and Environmental Protection, “Gheorghe Asachi” Technical University of Iasi, D. Mangeron 73, Iasi 700050, Romania

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The external and internal mass transfer of Penicillin G in the process of its enzymatic hydrolysis to 6-Aminopenicillanic acid under competitive and non-competitive inhibitions have been comparatively analyzed for a bioreactor with mobile bed vs. a stationary basket bioreactor, both with Penicillin amidase immobilized in Eupergit C. The Penicillin G mass transfer and hydrolysis enzymatic rates have been analyzed by means of the ratios’ values between the oxygen mass transfer coefficients, effectiveness factors, external mass flows and Penicillin G concentrations at the biocatalyst particle surface for the considered bioreactors. The results indicated that the bioreactor with mobile bed is more efficient especially for biocatalyst particles with diameter under 1.5 mm. For larger particles the performances of the two bioreactors become similar. Moreover, taking into consideration the external mass flow of Penicillin G and the number of enzymatic hydrolysis cycles, the basket bioreactor is recommended. The mathematical equations proposed are in good concordance with the experimental results, the average deviations varying from ±4.11% for the bioreactor with mobile bed of immobilized Penicillin amidase to ±5.03% for the basket bioreactor.

Keywords:Bioreactor;Diffusion;Immobilized Enzymes;Mass Transfer;Penicillin;Penicillin amidase

[References]

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Katchalski-Katzir K, Kraemer DM, J. Mol. Catal. B: Enzym., 10, 157 (2000)
Knezevi Z, Milosavi N, Bezbradica D, Jakovljevi Z, Prodanovi R, Biochem. Eng. J., 30, 269 (2006)
Lupasteanu AM, Galaction AI, Cascaval D, Rom. Biotechnol. Lett., 12, 3131 (2007)
Galaction AI, Matran RM, Turnea M, Blaga AC, Cascaval D, Chem. Eng. Commun., DOI: 10.1080/00986445.2013.819801. (2014)
Matran RM, Galaction AI, Blaga AC, Turnea M, Cascaval D, Environ. Eng. Manag. J., 12, 2261 (2013)
Gamarra A, Cuevas C, Lescano G, J. Ferm. Technol., 64, 25 (1986)
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Pitault I, Fongarland P, Koepke D, Mitrovic M, Ronze D, Forissier M, Chem. Eng. Sci., 60, 6240 (2007)
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Cascaval D, Turnea M, Galaction AI, Blaga AC, Biochem. Eng. J., 69, 113 (2012)
Galaction AI, Cascaval D, Turnea M, Folescu E, Bioprocess Biosyst. Eng., 27, 263 (2005)
Galaction AI, Lupasteanu AM, Cascaval D, Environ. Eng. Manag. J., 6, 101 (2007)
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Kumar R, Suresh AK, Shankar HS, J. Chem. Technol. Biotechnol., 66(3), 243 (1996)
Perry RH, Chilton CH, Chemical Engineers’ Handbook, McGraw-Hill, New York (1973)

[1] Bianchi D, Golini P, Bortolo R, Cesti P, Enzyme Microb. Technol., 18(8), 592 (1996)

[2] Katchalski-Katzir K, Kraemer DM, J. Mol. Catal. B: Enzym., 10, 157 (2000)

[3] Knezevi Z, Milosavi N, Bezbradica D, Jakovljevi Z, Prodanovi R, Biochem. Eng. J., 30, 269 (2006)

[4] Lupasteanu AM, Galaction AI, Cascaval D, Rom. Biotechnol. Lett., 12, 3131 (2007)

[5] Galaction AI, Matran RM, Turnea M, Blaga AC, Cascaval D, Chem. Eng. Commun., DOI: 10.1080/00986445.2013.819801. (2014)

[6] Matran RM, Galaction AI, Blaga AC, Turnea M, Cascaval D, Environ. Eng. Manag. J., 12, 2261 (2013)

[7] Gamarra A, Cuevas C, Lescano G, J. Ferm. Technol., 64, 25 (1986)

[8] Kolagerakis N, Behie LA, Bioprocess Biosyst. Eng., 17, 151 (1996)

[9] Pitault I, Fongarland P, Koepke D, Mitrovic M, Ronze D, Forissier M, Chem. Eng. Sci., 60, 6240 (2007)

[10] Sheelu G, Kavitha G, Fadnavis NW, J. Am. Oil. Chem. Soc., 85, 739 (2008)

[11] Teshima H, Ohashi Y, J. Chem. Eng. Jpn., 10, 70 (1977)

[12] Warna J, Ronnholm M, Salmi T, Keikko K, Computer-Aided Chem. Eng., 10, 1009 (2002)

[13] Cascaval D, Turnea M, Galaction AI, Blaga AC, Biochem. Eng. J., 69, 113 (2012)

[14] Galaction AI, Cascaval D, Turnea M, Folescu E, Bioprocess Biosyst. Eng., 27, 263 (2005)

[15] Galaction AI, Lupasteanu AM, Cascaval D, Environ. Eng. Manag. J., 6, 101 (2007)

[16] Torres-Bacete J, Arroyo M, Torres-Guzman R, de la Mata I, Castillon MP, Acebal C, Biotechnol. Lett., 22(12), 1011 (2000)

[17] Kumar R, Suresh AK, Shankar HS, J. Chem. Technol. Biotechnol., 66(3), 243 (1996)

[18] Warburton D, Dunnil P, Lilly MD, Biotechnol. Bioeng., 15, 13 (1973)

[19] Illanes A, Enzyme biocatalysis: Principles and applications, Springer, New York (2008)

[20] Kumar R, Suresh AK, Shankar HS, J. Chem. Technol. Biotechnol., 66(3), 243 (1996)

[21] Perry RH, Chilton CH, Chemical Engineers’ Handbook, McGraw-Hill, New York (1973)