Bioleaching of arsenic and heavy metals from mine tailings by pure and mixed cultures of Acidithiobacillus spp.

Van Khanh Nguyen; Mu Hyun Lee; Hyung Jun Park; Jong-Un Lee

Journal of Industrial and Engineering Chemistry, Vol.21, 451-458, January, 2015

DOI10.1016/j.jiec.2014.03.004 Export Citation

Bioleaching of arsenic and heavy metals from mine tailings by pure and mixed cultures of Acidithiobacillus spp.

Van Khanh Nguyen, Mu Hyun Lee, Hyung Jun Park, and Jong-Un Lee^†

Department of Energy and Resources Engineering, Chonnam National University, Gwangju 500-757, Republic of Korea

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Microbial removal of heavy metals in mine tailings from a Cu-Ag mine in the Philippines was investigated. Effect of bacterial strains on bioleaching and fractionation of heavy metals were also studied. Single and mixed cultures of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans were used separately to compare efficiency of metal solubilization. The results showed that mixed cultures were more efficient than each bacterium for Cu and As, while A. ferrooxidans demonstrated faster extraction efficiency for Mn and Zn. The results of sequential extraction for residues indicated that pure bacteria and mixed cultures mediated occurrence mode of heavy metals in different ways.

Keywords:Heavy metals;Arsenic;Bioleaching;Mine tailings;Acidithiobacillus ferrooxidans;Acidithiobacillus thiooxidans

[References]

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Chen SY, Lin JG, J. Hazard. Mater., 161(2-3), 893 (2009)
Krebs W, Brombacher C, Bosshard PP, Bachofen R, Brandl H, Fems Microbiol. Rev., 20, 605 (1997)
Akinci G, Guven DE, Desalination, 268(1-3), 221 (2011)
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Konishi Y, Kubo H, Asai S, Biotechnol. Bioeng., 39, 66 (1992)
Silverman MP, Ehrlich HL, Adv. Appl. Microbiol., 6, 181 (1964)
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Lizama HM, Suzuki I, Can. J. Microbiol., 37, 304 (1991)
Boon M, Snijder M, Hansford GS, Heijnen JJ, Hydrometallurgy, 33, 137 (1998)
Fowler TA, Crundwell FK, Appl. Environ. Microbiol., 64, 3570 (1998)
Liu YG, Zhou M, Zeng GM, Li X, Xu WH, Fan T, J. Hazard. Mater., 141(1), 202 (2007)
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Chen SY, Lin PL, Sep. Purif. Technol., 71(2), 178 (2010)
Tessier A, Campbell PGC, Bisson M, Anal. Chem., 51, 844 (1979)
Ahn JS, Park YS, Kim JY, Kim KW, Environ. Geochem. Health, 27, 147 (2005)
Stookey LL, Anal. Chem., 42, 779 (1970)
Lee JU, Kim SM, Kim KW, Kim IS, Chemosphere, 59, 147 (2005)
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[Referenced By]

[1] Mithras S, What is Bioleaching? Retrieved on 19 March 2014 from http://www.wisegeek.com/what-is-bioleaching.htm.

[2] Chen SY, Lin JG, J. Hazard. Mater., 161(2-3), 893 (2009)

[3] Krebs W, Brombacher C, Bosshard PP, Bachofen R, Brandl H, Fems Microbiol. Rev., 20, 605 (1997)

[4] Akinci G, Guven DE, Desalination, 268(1-3), 221 (2011)

[5] Chen SY, Lin JG, J. Hazard. Mater., 82(1), 77 (2001)

[6] Bosecker K, Fems Microbiol. Rev., 20, 591 (1997)

[7] Konishi Y, Kubo H, Asai S, Biotechnol. Bioeng., 39, 66 (1992)

[8] Silverman MP, Ehrlich HL, Adv. Appl. Microbiol., 6, 181 (1964)

[9] Free ML, Oolman T, Nagpal S, Dahlstroom DA, in: Smith RW, Misra M (Eds.), Mineral Bioprocessing, The Minerals, Metals and Materials Society, Warrendale, p. 485. (1991)

[10] Lizama HM, Suzuki I, Can. J. Microbiol., 37, 304 (1991)

[11] Boon M, Snijder M, Hansford GS, Heijnen JJ, Hydrometallurgy, 33, 137 (1998)

[12] Fowler TA, Crundwell FK, Appl. Environ. Microbiol., 64, 3570 (1998)

[13] Liu YG, Zhou M, Zeng GM, Li X, Xu WH, Fan T, J. Hazard. Mater., 141(1), 202 (2007)

[14] Liu YG, Zhou M, Zeng GM, Wang X, Li X, Fan T, Xu WH, Bioresour. Technol., 99(10), 4124 (2008)

[15] Fu B, Zhou H, Zhang R, Qiu G, Int. Biodeterior. Biodegrad., 62, 109 (2008)

[16] Zhang YS, Qin WQ, Wang J, Zhen SJ, Yang CR, Zhang JW, Nai SS, Qiu GZ, Trans. Nonferrous Met. Soc. China, 18, 1491 (2008)

[17] Zhang J, Zhang X, Ni Y, Yang X, Li H, Process Biochem., 42, 1265 (2007)

[18] Korean Ministry of Environment, Soil environment standard test, in: Soil Environment Preservation Act, Korean Ministry of Environment (2012)

[19] DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH), Growth mediums of Acidithiobacillus spp., retrieved on 19 March 2014, from http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium271.pdf

[20] NCIMB (National Collection of Industrial, Food and Marine Bacteria), NCIMB Culture Media v2.0, accessed on 19 March 2014 from http://www.ncimb.com/.

[21] Paolo V, Emma B, Mario P, Vincenzo B, Piera V, Nicola O, J. Gen. Appl. Microbiol., 35, 71 (1989)

[22] Chen SY, Lin PL, Sep. Purif. Technol., 71(2), 178 (2010)

[23] Tessier A, Campbell PGC, Bisson M, Anal. Chem., 51, 844 (1979)

[24] Ahn JS, Park YS, Kim JY, Kim KW, Environ. Geochem. Health, 27, 147 (2005)

[25] Stookey LL, Anal. Chem., 42, 779 (1970)

[26] Lee JU, Kim SM, Kim KW, Kim IS, Chemosphere, 59, 147 (2005)

[27] Perin G, Fabris R, Manente S, Wagener RA, Hamacher C, Scotto S, Water Res., 31, 3017 (1997)