Preparation and drug release behavior of pH-responsive bovine serum albumin-loaded chitosan microspheres

Xue Zou; Xiaowen Zhao; Lin Ye; Qi Wang; Hao Li

Journal of Industrial and Engineering Chemistry, Vol.21, 1389-1397, January, 2015

DOI10.1016/j.jiec.2014.06.012 Export Citation

Preparation and drug release behavior of pH-responsive bovine serum albumin-loaded chitosan microspheres

Xue Zou, Xiaowen Zhao^†, Lin Ye, Qi Wang¹, and Hao Li¹

State Key Laboratory of Polymer Materials Engineering of China, Polymer Research Institute of Sichuan University, Chengdu, China
¹State Key Laboratory of Oral Diseases, West China Hospital of Stomatology of Sichuan University, Chengdu, China

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A novel inverse emulsion-ionic crosslinking approach was adopted to prepare pH-responsive chitosan microspheres and effect of preparation technique, such as chitosan concentration, emulsifier concentration, water/oil ratio and homogenization speed, on the morphology and property of the microspheres were investigated. The chitosan microspheres with spherical in shape, smooth surface, high productivity and promising pH responsiveness were obtained. On this basis, bovine serum albumin (BSA)-loaded chitosan microspheres was prepared to test the drug release behavior in buffers with different pH values. BSA-chitosan microspheres with particle sizes in range from 5 to 10μm released BSA slowly in neutral medium and released rapidly in acidic medium, and exhibited obviously pH-responsive release behavior. Korsmeyer-Peppas model can be used for describing BSA release kinetics from chitosan microspheres, which corresponded with a Fickian release behavior, and the diffusion through the swelling of chitosan microspheres was the main factor in controlling BSA release.

Keywords:Chitosan (CS);Microspheres;Preparation technique;pH-responsive;Swelling behavior;Drug release behavior

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[Referenced By]

[1] Gupta P, Vermani K, Garg S, Drug. Discov. Today, 7, 569 (2002)

[2] Lee SM, Chen H, Dettmer CM, O'Halloran TV, Nguyen ST, J. Am. Chem. Soc., 129(49), 15096 (2007)

[3] Vasconcelos T, Sarmento B, Costa P, Drug Discov. Today, 12, 1068 (2007)

[4] Valet G, Raffael A, Moroder L, Naturwissenschaften, 68, 265 (1981)

[5] Ganta S, Devalapally H, Shahiwala A, J. Control. Release, 126, 187 (2008)

[6] Andresen TL, Jensen SS, Jorgensen K, Prog. Lipid Res., 44, 68 (2005)

[7] Allen TM, Cullis PR, Science, 303, 1818 (2004)

[8] Deshmukh VN, Singh SP, Sakarkar DM, Int. J. Pharm. Tec. Res., 1, 159 (2009)

[9] Taillefer J, Jones MC, Brasseur N, J. Pharm. Sci., 89, 52 (2000)

[10] Kwon YJ, James E, Shastri NP, Natl. Acad. Sci. USA, 102, 18264 (2005)

[11] Rizi K, Green RJ, Donaldson M, J. Pharm. Sci., 100, 566 (2011)

[12] Lynn DM, Amiji MM, Langer R, Angew. Chem.-Int. Edit., 40, 1707 (2001)

[13] Li GL, Liu G, Kang ET, Neoh KG, Yang XL, Langmuir, 24(16), 9050 (2008)

[14] Zhang J, Zhang W, Wang Y, Adv. Synth. Catal., 350, 2065 (2008)

[15] Lv PP, Ma YF, Yu R, Mol. Pharm., 9, 1736 (2012)

[16] Liu L, Yang JP, Ju XJ, Soft Matter, 7, 4821 (2011)

[17] Wei W, Ma GH, Wang LY, Acta Biol., 6, 205 (2010)

[18] Yao KD, Xu MX, Yin YJ, Polym. Int., 39, 333 (1996)

[19] Sun P, Li P, Li YM, J. Biomed. Mater. Res. B, 97, 175 (2011)

[20] Jameela SR, Kumary TV, Lal AV, J. Control. Release, 52, 17 (1998)

[21] Maleki A, Kjoniksen AL, Nystrom B, Carbohydr. Res., 342, 2776 (2007)

[22] Van Wijk A, Siebum A, Schoevaart R, Carbohydr. Res., 341, 2921 (2006)

[23] Li P, Wang Y, Carbohydr. Res., 346, 801 (2011)

[24] Muzzarelli C, Tosi G, Francescangeli O, Carbohydr. Res., 338, 2247 (2003)

[25] Garud N, Garud A, Trop. J. Pharm. Res., 11, 577 (2012)

[26] Mi FL, Kuan CY, Shyu SS, Carbohydr. Polym., 41, 389 (2000)

[27] Gupta KC, Jabrail FH, Carbohydr. Res., 341, 744 (2006)

[28] Jiang DS, Long SY, Huang J, Biochem. Eng. J., 25, 15 (2005)

[29] Shu XZ, Zhu KJ, Song W, Int. J. Pharm., 212, 19 (2001)

[30] Ko JA, Park HJ, Hwang SJ, Int. J. Pharm., 249, 165 (2002)

[31] Lin WC, Yu DG, Yang MC, Colloids Surf. B: Biointerfaces, 44, 143 (2005)

[32] Wang LYG, Zhou YH, Colloids Surf. B: Biointerfaces, 50, 126 (2006)

[33] Niu XF, Feng QL, Wang MB, Guo XD, Zheng QX, J. Microencapsul., 26(4), 297 (2009)

[34] Pawlak A, Mucha A, Thermochim. Acta, 396(1-2), 153 (2003)

[35] Rivero S, Garcia MA, Pinotti A, Carbohydr. Polym., 82, 270 (2010)

[36] Quinones JP, Gothelf KV, Kjems JJ, Biomater. Tissue Eng., 3, 164 (2013)

[37] Berger J, Reist M, Mayer JM, Eur. J. Pharm. Biopharm., 57, 19 (2004)

[38] Vaghani SS, Patel MM, Satish CS, Carbohydr. Res., 347, 76 (2012)