Calcium carbonate-mineralized polymer nanoparticles for pH-responsive robust nanocarriers of docetaxel

Byung Joo Kim; Kyung Hyun Min; Gyu Ha Hwang; Hong Jae Lee; Seo Young Jeong; Eun-Cheol Kim; Sang Cheon Lee

Macromolecular Research, Vol.23, No.1, 111-117, January, 2015

DOI10.1007/s13233-015-3020-6 Export Citation

Calcium carbonate-mineralized polymer nanoparticles for pH-responsive robust nanocarriers of docetaxel

Byung Joo Kim, Kyung Hyun Min, Gyu Ha Hwang, Hong Jae Lee¹, Seo Young Jeong, Eun-Cheol Kim², and Sang Cheon Lee^{1, †}

Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea
¹Department of Maxillofacial Biomedical Engineering & Institute of Oral Biology, School of Dentistry,, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea
²Department of Maxillofacial Tissue Regeneration and Research Center for Tooth and Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Korea

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A calcium carbonate (CaCO3)-mineralized core-shell-corona micelle was evaluated as an intracellular nanocarrier of docetaxel (DTX). The polymer micelle consisting of the poly(ethylene glycol) coronal, the anionic poly(L-aspartic acid) middle shell, and the DTX-loaded hydrophobic poly(L-phenylalanine) core was used as a nanotemplate for CaCO3 mineralization was performed by initial electrostatic localization of calcium ions at the anionic PAsp middle shells, and the subsequent addition of carbonate anions to trigger the growth of CaCO3 around the PAsp middle shells. Even after mineralization, the micelle size and the spherical morphology were maintained. The CaCO3-mineralized micelles exhibited enhanced stability against micelle-disrupting surfactants and the serums. The mineralized CaCO3 layer could suppress DTX release efficiently at extracellular pH (7.4) but dissolve at an intracellular endosomal pH (pH ~ 5.0) to facilitate the DTX release. These results reveal the promising potential of CaCO3-mineralized polymer micelle as a stable and effective nanocarrier system in tumor therapy.

Keywords:mineralization;pH-responsive;calcium carbonate;docetaxel;intracellular release

[References]

Han HS, Lee J, Kim HR, Chae SY, Kim M, Saravanakumar G, Yoon HY, You DG, Ko H, Kim K, Kwon IC, Park JC, Park JH, J. Control. Release, 168, 105 (2013)
Fantechi E, Innocenti C, Zanardelli M, Fittipaldi M, Falvo E, Carbo M, Shullani V, Mannelli LDC, Ghelardini C, Ferretti AM, Ponti A, Sangregorio C, Ceci P, ACS Nano, 8, 4705 (2014)
Sivasubramanian M, Thambi T, Park JH, Carbohydr. Polym., 97, 643 (2013)
Fan KL, Cao CQ, Pan YX, Lu D, Yang DL, Feng J, Song LN, Liang MM, Yan XY, Nat. Nanotechnol., 7(7), 459 (2012)
Cha EJ, Sun IC, Lee SC, Kim K, Kwon IC, Ahn CH, Macromol. Res., 20(3), 319 (2012)
Schmidt HT, Gray BL, Wingert PA, Ostafin AE, Chem. Mater., 16, 4942 (2004)
Sugawara A, Yamane S, Akiyoshi K, Macromol. Rapid Commun., 27(6), 441 (2006)
Addadi L, Weiner S, Proc. Natl. Acad. Sci. U.S.A., 50, 49 (1986)
Han S, Han HS, Lee SC, Kang YM, Kim I, Park JH, J. Mater. Chem., 21, 7996 (2011)
Xu Q, Tanaka Y, Czernuszka JT, Biomaterials, 28, 2687 (2007)
Lee HJ, Kim SE, Kwon IK, Park C, Kim C, Yang J, Lee SC, Chem. Commun., 46, 377 (2010)
Lee SU, Min KH, Jeong SY, Bae H, Lee SC, Chem. Asian J., 8, 3222 (2013)
Min KH, Lee HJ, Kim K, Kwon IC, Jeong SY, Lee SC, Biomaterials, 33, 5788 (2012)
Rim HP, Min KH, Lee HJ, Jeong SY, Lee SC, Angew. Chem. Int. Ed., 50, 8853 (2011)
Smith BL, Schaffer TE, Viani M, Thompson JB, Frederick NA, Kindt J, Belcher A, Stucky GD, Morse DE, Hansma PK, Nature, 399(6738), 761 (1999)
Volodkin DV, Larionova NI, Sukhorukov GB, Biomacromolecules, 5(5), 1962 (2004)
Kreft O, Prevot M, Mohwald H, Sukhorukov GB, Angew. Chem., Int. Ed., 46, 5605 (2007)
Choi GH, Min KH, Lee SC, Macromol. Res., 22(3), 329 (2014)
Lee SJ, Min KH, Lee HJ, Koo AN, Rim HP, Jeon BJ, Jeong SY, Heo JS, Lee SC, Biomacromolecules, 12(4), 1224 (2011)
Harada A, Kataoka K, Macromolecules, 31(2), 288 (1998)
Goss SL, Lemons KA, Kerstetter JE, Bogner RH, J. Pharm. Pharmacol., 59, 1485 (2007)
Nido PJD, Glynn P, Buenaventura P, Salama G, Koretsky AP, Am. J. Physiol. Heart Circ. Physiol., 728e41, 274 (1998)
Fonteriz RI, Moreno SDIA, Lobaton CD, Montero M, Alvarez J, Cell Calcium, 61e9, 48 (2010)

[Referenced By]

[1] Han HS, Lee J, Kim HR, Chae SY, Kim M, Saravanakumar G, Yoon HY, You DG, Ko H, Kim K, Kwon IC, Park JC, Park JH, J. Control. Release, 168, 105 (2013)

[2] Fantechi E, Innocenti C, Zanardelli M, Fittipaldi M, Falvo E, Carbo M, Shullani V, Mannelli LDC, Ghelardini C, Ferretti AM, Ponti A, Sangregorio C, Ceci P, ACS Nano, 8, 4705 (2014)

[3] Sivasubramanian M, Thambi T, Park JH, Carbohydr. Polym., 97, 643 (2013)

[4] Fan KL, Cao CQ, Pan YX, Lu D, Yang DL, Feng J, Song LN, Liang MM, Yan XY, Nat. Nanotechnol., 7(7), 459 (2012)

[5] Cha EJ, Sun IC, Lee SC, Kim K, Kwon IC, Ahn CH, Macromol. Res., 20(3), 319 (2012)

[6] Schmidt HT, Gray BL, Wingert PA, Ostafin AE, Chem. Mater., 16, 4942 (2004)

[7] Sugawara A, Yamane S, Akiyoshi K, Macromol. Rapid Commun., 27(6), 441 (2006)

[8] Addadi L, Weiner S, Proc. Natl. Acad. Sci. U.S.A., 50, 49 (1986)

[9] Han S, Han HS, Lee SC, Kang YM, Kim I, Park JH, J. Mater. Chem., 21, 7996 (2011)

[10] Xu Q, Tanaka Y, Czernuszka JT, Biomaterials, 28, 2687 (2007)

[11] Lee HJ, Kim SE, Kwon IK, Park C, Kim C, Yang J, Lee SC, Chem. Commun., 46, 377 (2010)

[12] Lee SU, Min KH, Jeong SY, Bae H, Lee SC, Chem. Asian J., 8, 3222 (2013)

[13] Min KH, Lee HJ, Kim K, Kwon IC, Jeong SY, Lee SC, Biomaterials, 33, 5788 (2012)

[14] Rim HP, Min KH, Lee HJ, Jeong SY, Lee SC, Angew. Chem. Int. Ed., 50, 8853 (2011)

[15] Smith BL, Schaffer TE, Viani M, Thompson JB, Frederick NA, Kindt J, Belcher A, Stucky GD, Morse DE, Hansma PK, Nature, 399(6738), 761 (1999)

[16] Volodkin DV, Larionova NI, Sukhorukov GB, Biomacromolecules, 5(5), 1962 (2004)

[17] Kreft O, Prevot M, Mohwald H, Sukhorukov GB, Angew. Chem., Int. Ed., 46, 5605 (2007)

[18] Choi GH, Min KH, Lee SC, Macromol. Res., 22(3), 329 (2014)

[19] Lee SJ, Min KH, Lee HJ, Koo AN, Rim HP, Jeon BJ, Jeong SY, Heo JS, Lee SC, Biomacromolecules, 12(4), 1224 (2011)

[20] Harada A, Kataoka K, Macromolecules, 31(2), 288 (1998)

[21] Goss SL, Lemons KA, Kerstetter JE, Bogner RH, J. Pharm. Pharmacol., 59, 1485 (2007)

[22] Nido PJD, Glynn P, Buenaventura P, Salama G, Koretsky AP, Am. J. Physiol. Heart Circ. Physiol., 728e41, 274 (1998)

[23] Fonteriz RI, Moreno SDIA, Lobaton CD, Montero M, Alvarez J, Cell Calcium, 61e9, 48 (2010)