Organic single crystals have attracted great attention because of their advantages of high charge-carrier mobility, high chemical purity, and potential for flexible optoelectronic devices. However, their intrinsic properties of sensitive to organic solvent and fragile result in a difficulty in the fabrication of the organic crystal-based devices. In this work, a simple and non-destructive technique of template stripping is employed to fabricate single-crystal-based organic light-emitting devices (OLEDs). Efficient and uniform carrier injection induced by an improved contact between crystals and both top and bottom electrodes is realized, so that a homogeneous and bright electroluminescence (EL) are obtained. Highly polarized EL and even white emission is also observed. Moreover, the crystal-based OLEDs exhibit good flexibility, and keep stable EL under a small bending radius and after repeated bending. It is expectable that this technique would support broad applications of the organic single crystals in the crystal-based optoelectronic devices.