A facile method was developed to prepare biodegradable nanocomposite films. Ag nanoparticies stabilized by an NH2-terminated fourth generation poly(amido amine) dendrimer (DENAgNPs) were covalently immobilized on 2,2,6,6-tetramethylpiperidine-1-oxyl radical-oxidized nanofibrillated cellulose (NFC) by using a condensing agent for amide bond formation between NFC and DENAgNPs. Subsequently, the films with different concentrations of DENAgNPs were prepared by filtrating DENAgNPs-NFC suspensions on filter membranes, and the dried films were characterized. As-prepared films possessed the high catalytic efficiency to decoloration of Rhodamine B in water and the excellent antibacterial performance against both Gram-positive and Gram-negative bacteria on agar plates. Thus, this novel concept of film-type nanocomposite reactor demonstrates that it is possible to prepare inexpensive catalytic and bactericidal-active films with good selectivity by easy prospective approach for realizing the environmental conservation and innovation. (C) 2014 Elsevier B.V. All rights reserved.