Flowers have distinct glycerolipid composition, yet its floral organ-specific profile remains elusive in Arabidopsis whose flowers are too tiny to dissect different floral organs. Here, we employed known floral homeotic mutants agamous-1 (ag-1) and apetala3-3 (ap3-3) to facilitate sample preparation enriched in different floral organs. The result of analysis on different polar glycerolipid classes and their fatty acid composition demonstrated that flowers of ap3-3 and ag-1 have distinct glycerolipid composition from that of wild type. Moreover, distinct set of glycerolipid biosynthetic genes is expressed in these mutants by qRT-PCR gene expression analysis. These data suggest that glycerolipid profile is distinct among different floral organs of Arabidopsis thaliana. (C) 2014 Elsevier Inc. All rights reserved.