Biochemical and Biophysical Research Communications, Vol.452, No.1, 32-35, 2014
Screening of recombinant Escherichia coli using activation of green fluorescent protein as an indicator
A novel cloning vector that can be used to identify recombinant Escherichia coli colonies by activation of the green fluorescent protein gene (GFP) was constructed. Screening using the vector does not require special reagents. The recombinant plasmid activates GFP, and the rate of false-positive results is low. (C) 2014 Elsevier Inc. All rights reserved.