This study was conducted to investigate the long-term impact of influent phosphorus concentration on denitrifying phosphorus removal and N2O production during denitrifying phosphorous removal process. The results showed that, denitrifying phosphate accumulating organisms (DPAOs) could become dominant populations quickly in anaerobic/anoxic SBR by providing optimum cultivating conditions, and the reactor performed well for denitrifying phosphorus removal. The influent phosphorus concentration significantly affected anaerobic poly-beta-hydroxyalkanoates (PHA) synthesis, denitrifying phosphorus removal, and N2O production during the denitrifying phosphorus removal process. As the influent phosphorus concentration was more than 20 mg L-1, the activity of DPAOs began to be inhibited due to the transformation of the available carbon source type. Meanwhile, N2O production was inhibited with the mitigation of anoxic NO2-N accumulation. Adoption of a modified feeding could enhance denitrifying phosphorus removal and inhibit N2O production during denitrifying phosphorous removal processes. (C) 2014 Elsevier Ltd. All rights reserved.