An accurate measurement or estimation of the volumetric mass transfer coefficient k(L)a is crucial for the design, operation, and scale up of bioreactors. Among different physical and chemical methods, the classical dynamic method is the most widely applied method to simultaneously estimate both k(L)a and cell's oxygen utilization rate. Despite several important follow-up articles to improve the original dynamic method, some limitations exist that make the classical dynamic method less effective under certain conditions. For example, for the case of high cell density with moderate agitation, the dissolved oxygen concentration barely increases during the re-gassing step of the classical dynamic method, which makes k(L)a estimation impossible. To address these limitations, in this work we present an improved dynamic method that consists of both an improved model and an improved procedure. The improved model takes into account the mass transfer between the headspace and the broth; in addition, nitrogen is bubbled through the broth when air is shut off. The improved method not only enables a faster and more accurate estimation of k(L)a, but also allows the measurement of k(L)a for high cell density with medium/low agitation that is impossible with the classical dynamic method. Scheffersomyces stipitis was used as the model system to demonstrate the effectiveness of the improved method; in addition, experiments were conducted to examine the effect of cell density and agitation speed on k(L)a. (C) 2014 Wiley Periodicals, Inc.