Label-free detection of glucose molar concentration was carefully studied with a device which was designed to modulate temperature in a local area by Joule heating from room temperature to human body temperature. Very small amount of glucose concentration up to sub-millimolar range was detected based on the glucose concentration dependent electrical resistance change with the temperature modulation since it allowed more electrical conductivity enhancement for lower glucose concentration. (C) 2014 Published by Elsevier B.V.