Electropolymerisation of folk acid (FA) was performed in different media in order to optimise the best conditions for the formation of a stable poly(folic acid) (PFA) film. Different substrates were also used for PFA deposition. The electrochemical stability of the PFA film was insufficient; therefore, graphene-chitosan (G-Chit) composite was deposited on the top of PFA film in order to prevent it from washing out of the electrode surface. A possible electropolymerisation mechanism was proposed. The obtained films were characterised microscopically and electrochemically applying atomic force microscopy (AFM) and electrochemical methods such as cyclic voltammetry (CV), square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) in different media. The best film was obtained when PFA was electrosynthesised from 0.1 mol L-1 KCl/HCl solution, pH 2.0, covered with G-Chit composite film. The best electrochemical behaviour of such electrode was in the same buffer solution. This electrode will be further applied to (bio)sensor construction. (C) 2014 Elsevier Ltd. All rights reserved.