Polyacrylamide (PAM)/reduced graphene oxide ( rGO) nanocomposite (PAM/rGO) with rGO nano s heets as cross-linkers were synthesized via electropolymerizing acrylamide in an aqueous suspension of GO containing sodium nitrate and potassium persulfate. The PAM/rGO was characterized with X-ray diffraction (XRD), Fourier transfer infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). XRD patterns of the PAM/rGO indicated that rGO was fully exfoliated into individual sheets in the polymer matrix. SEM study exhibits the highly uniform coverage of PAM/rGO membrane on the GCE and its fibrous nanostructure. The electrochemical characteristics of the PAM/rGO modified glassy carbon electrode (GCE) were investigated with cyclic voltammetry (CV), suggesting the extraordinary electrocatalytic effect of PAM/rGO/GCE towards the electro-oxidation of UA and DA. The PAM/rG0/ GCE behaved linearly to DA and UA in the concentration range of 3 x 10(7) to 5 x 10(5) M and 1 x 10(6) to 5 x 10(5) M with a detection limit of 1 x 10(7) M and 5 x 10(7) M, respectively. The proposed method was successfully utilized for simultaneous determination of UA and DA in real samples. (C) 2014 Elsevier Ltd. All rights reserved.