Aminophenylboronic acid moieties were covalently grafted onto mercaptobenzoic acid moieties, and glucose oxidase was then immobilized through boronic acid-diol specific recognition to form a pH-sensitive electrosensor switching between pH 5.8 and pH 8.0 base solution. Using potassium ferricyanide as electroactive probe, the response was intensified in acidic condition while hindered in alkaline condition. The sharp and stable contrast in current was performed alternately upon the change of pH like a "pH switch". In the presence of glucose, the response to glucose was further amplified catalytically by glucose oxidase on "ON" state, while electron transfer was inhibited on "OFF" state. Furthermore, when our sensor was on "ON" state, it showed a good linearity ranging from 0 to 30 mu mol L-1 of glucose, with a detection limit of 348 nmol L-1 (S/B = 3) and a dynamic range extending to 50 mu mol L-1. Glucose-responsive, pH-switchable and catalytically-amplified, our biosensor provided a new method for the detection of glucose in the form of pH switch in human serum sample, and was promising to more complicated environment. (C) 2014 Elsevier Ltd. All rights reserved.