Experiments with transesterification of rapeseed oil in supercritical alcohols (methanol, ethanol and 1-propanol) were carried out in a batch reactor at various reaction temperatures (250-350 degrees C), working pressure (8-12 MPa), reaction time, and constant 42:1 alcohol to oil molar ratio. Influence of different alcohols and reaction conditions on biodiesel yield was investigated using linear multiple regression models. Temperature had the highest impact on yields, followed by reaction time and pressure. With increased molecular weight of alcohols, relative importance of temperature for explanation of yields decreased and relative importance of time and pressure increased. Economic assessment has revealed that transesterification in supercritical methanol has the lowest direct material and energy costs. Yield has crucial impact on process economics. Direct costs decrease with increase in biodiesel yields. Even at very low prices of oil feedstock the lowest cost is achieved at the highest yield. (C) 2014 Elsevier Ltd. All rights reserved.