Polymer-coated magnetic particles (MPs) were prepared to study the binding of fluorescence dye on the surface and its H2O2-induced release. For this goal, multilayer films were prepared by layer-by-layer deposition of shikimic acid-appended poly(allylamine hydrochloride) (SA-PAH) and poly(styrenesulfonate) (PSS) on the surface of MPs. 3-(Dansylamino)phenylboronic acid (DPBA) was loaded on the MPs through boronate ester bonding between SA-PAH and DPBA. DPBA was released from the MPs in response to H2O2 as a result of breakage of the boronate ester bond by an oxidative reaction with H2O2. DPBA release was dependent on the H2O2 concentration. For example, 65% and 93% of the DPBA was released from (SA-PAH/PSS)(4)SA-PAH film-coated MPs in 30 min after the addition of 0.1 and 0.5 mM H2O2, respectively. In addition, the multilayer film-coated MPs were further modified by using glucose oxidase (GOx) to develop glucose-induced release systems. GOx-modified MPs released DPBA in response to 0.1 mM D-glucose as a result of H2O2 generation through a GOx-catalyzed oxidation reaction of D-glucose. The results suggest a potential use of the multilayer film-coated MPs in the development of H2O2- and/or glucose-sensitive drug delivery systems. (C) 2014 Elsevier Inc. All rights reserved.