We examine the combined influence of the intensity of pressure driven background flow and the frequency of the applied field on the continuous-flow dielectrophoretic trapping behavior of micro-particles within a micro-channel. Using an embedded interdigitated electrode array, we find that the measured trapping percentage over a continuous frequency range exhibits several curious effects which are strongly dependent on the flow intensity, including an apparent shift of the cross-over frequency and low-frequency dispersion. A numerical and theoretical model accounting for the combined effects of pressure-driven flow, dielectrophoresis and alternating-current electro-osmosis on the equation of motion for the particle is used to qualitatively describe the main experimental results. (C) 2014 Elsevier Inc. All rights reserved.