The purpose of this paper is to summarize experimental data of CdS/L-cystein nanopartides (NPs) colloid solutions optical properties as function of 3-component mixture (Cd2+-S2--L-cys) content. The NPs synthesis was carried out at pH 11 in the wide composition range based on literature data. The influence of the solutions composition on the colloids stability and the CdS/I-cys NPs size was monitored by absorption and fluorescence spectra observation at room temperature and studied by TEM. A polynomial model was used for the responses produced from Scheffe's simplex lattice design with fourth degree terms. The paper also compares the results of two statistical-experimental methods, namely 2D Scheffe's simplex-lattice design and 3D-plot by STATISTICA v6 program. Both models were found to be adequate for prediction the optical properties of the solutions but with various approximation levels. (C) 2014 Elsevier Ltd. All rights reserved.