The gene encoding an endo alpha-1,4 polygalactosaminidase (P-GalNase) from Pseudomonas sp. 881 was cloned into Escherichia coli DH1 and MV1184 by using charomid 9-36 and a pUC vector, and then sequenced. The gene encoding the enzyme P-GalNase consisted of 882 bp encoding 294 amino acid residues including a leader sequence. The deduced amino acid sequence did not show any extensive homology with sequences of other glycanases listed in the SWISS-PROT (GenBank) data bank, but we could observe some homology between the middle segment of the P-GalNase sequence and the conserved sequences of chitinases and related glycosidases.