Two new anthraquinones are described which were produced by stationary cultures of Fusarium oxysporum isolated from the roots of diseased citrus trees. They are 2-acetyl-3,8-dihydroxy- or 3-acetyl-2,8-dihydroxy-6-methoxy-anthraquinone and 2-(1-hydroxyethyl)-3,8-dihydroxy- or 3-(1-hydroxyethyl)-2,8-dihydroxy-6-methoxy-anthraquinone. These compounds differ from most other microbial anthraquinones in that they lack hydroxyl substituents at the 1,4 positions, and have an acetyl or 1-hydroxyethyl group at the 2 or 3 positions. This is the first report of anthraquinone metabolites produced by F. oxysporum.