This study was conducted to find a solution for the treatment of simulated industrial paint effluent using natural material, Strychnos potatorum, as a coagulant. The coagulation capacity was evaluated for a liter of effluent using standard jar test apparatus. The FTIR spectrum confirmed the existence of various functional groups in S. potatorum. The residence of proteins tied with coagulant treatment were determined and analyzed over SDS PAGE. The predominance of determining variables such as eluent type (water, NaCl, KCl), eluent concentration (1-5N), coagulant dosage (1-10 g), coagulant volume (20-100 mL), initial pH (5-11), and initial effluent concentration (3100, 4224, 5650, 6258, and 7693 mg/L named as sample number 1 to 5, respectively) were valued in terms of color, COD, and turbidity. The choicest outcome of the treatment was identified as, 2 g of S. potatorum, 3N NaCl as an eluent, 100 mL of coagulant volume, and the actual pH of the sample (7.2-7.8). It was found that the removal efficiency increased as the pollution load swelled. The results were matched with conventional coagulant alum. The amount of sludge formation was appreciably lower for natural coagulant. The results affirmed that the S. potatorum could act as an alternate solution for the paint effluent treatment.