Three-dimensionally ordered macroporous cross-linked polystyrene loaded with quaternary ammonium groups (3DOM CLPS-QA) was prepared for using as a novel adsorbent. The performance of 3DOM adsorbent was evaluated by adsorbing salicylic acid (SA) in aqueous solution. Adsorption isotherm and kinetics were investigated under different temperatures (293 K, 303 K, 313 K). The rising temperature led to a significant increase in the adsorption capacity towards SA, and 3DOM CLPS-QA exhibited a high adsorption capacity (317.86 mg/g when the initial concentration of SA was 500 mg/L at 303 K). Moreover, the effect of solution pH on adsorption indicated that the charge interaction was the dominating driving force for the adsorption of SA onto 3DOM CLPS-QA from the aqueous solution. The 3DOM adsorbent was also reusable and could be regenerated by 0.1 M hydrochloric acid solution. After reusing for six times, the adsorption capacity still maintained about 80% of the original capability.