Human hair keratin-based biofilm for potent application to periodontal tissue regeneration

Hanna Lee; Yu-Shik Hwang; Hyo-Seol Lee; Suji Choi; So Yeon Kim; Ji-Hoi Moon; Jae Hyung Kim; Kwang Chul Kim; Dong-Wook Han; Hyun-Jin Park; Hojae Bae

Macromolecular Research, Vol.23, No.3, 300-308, March, 2015

DOI10.1007/s13233-015-3036-y Export Citation

Human hair keratin-based biofilm for potent application to periodontal tissue regeneration

Hanna Lee, Yu-Shik Hwang¹, Hyo-Seol Lee², Suji Choi¹, So Yeon Kim¹, Ji-Hoi Moon¹, Jae Hyung Kim², Kwang Chul Kim¹, Dong-Wook Han³, Hyun-Jin Park, and Hojae Bae^{4, †}

Department of Food Biochemistry and Engineering, Korea University, Seoul, 136-713, Korea
¹Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea
²Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea
³Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Korea
⁴Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea

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For the treatment of periodontitis, local delivery of antibiotics and their sustained release are preferable to enhance microbial susceptibility and to reduce possible side effects occurrence in systemic drug delivery. To address these issues, antibiotic loaded polymeric matrices implantable into periodontal pocket have been commercially used in dentistry and oral medicine. Recently, keratin has been drawing attention as a natural polymer for its ability to mediate cell behavior with minor or no immunogenicity enabling further process towards autologous implantation. In this study, human hair keratin was extracted with a cocktail of reducing agents, and antibiotic eluting keratin-based biofilms were fabricated. Physicochemical analysis and release test showed proper physical stability and sustained release of the loaded antibiotics. In addition, the released antibiotic suppressed the growth of various types of oral bacteria including porphyromonas gingivalis. Cellular interaction studies showed that human oral epithelial cell, human gingival fibroblast and periodontal ligament cells proliferated and guided well on biofilms. This study propose that antibiotic eluting keratin-based biofilms are provisional device for the treatment of chronic periodontitis offering advantages such as local controlled drug delivery and biocompatibility, and human hair keratin is able to be a good biomaterial for the potent applications to tissue engineering and regenerative medicine.

Keywords:human hair keratin;film;drug-eluting;periodontal tissue

[References]

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[Referenced By]

[1] Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, Aboyans V, Lancet, 380, 2163 (2013)

[2] Smith AW, Adv. Drug Deliv. Rev., 57, 1539 (2005)

[3] Wang YX, Robertson JL, Spillman WB, Claus RO, Pharm. Res., 21, 1362 (2004)

[4] Siegle RJ, McCoy JP, Schade W, Swanson NA, Arch Dermatol., 120, 183 (1984)

[5] Chu PK, Chen J, Wang L, Huang N, Mater. Sci. Eng. R-Rep., 36, 143 (2002)

[6] Badylak SF, Gilbert TW, Semin. Immunol., 20, 109 (2008)

[7] Reichl S, Biomaterials, 30, 6854 (2009)

[8] Windoffer R, Beil M, Magin TM, Leube RE, J. Cell Biol., 194, 669 (2011)

[9] Langbein L, Yoshida H, Praetzel-Wunder S, Parry DA, Schweizer J, J. Invest. Dermatol., 130, 55 (2009)

[10] Heid HW, Moll I, Franke WW, Differentiation, 37, 137 (1988)

[11] Alonso L, Fuchs E, J. Cell Sci., 119, 391 (2006)

[12] Richter JR, de Guzman RC, Van Dyke ME, Biomaterials, 32, 7555 (2011)

[13] Reichl S, Borrelli M, Geerling G, Biomaterials, 32, 3375 (2011)

[14] Yamauchi C, Okazaki W, Yoshida T, Karasawa A, Biol. Pharm. Bull., 31, 994 (2008)

[15] Lee H, Noh K, Lee SC, Kwon IK, Han DW, Lee IS, Hwang YS, Tissue Eng. Regen. Med., 11, 255 (2014)

[16] Nakamura A, Arimoto M, Takeuchi K, Fujii T, Biol. Pharm. Bull., 25, 569 (2002)

[17] Schrooyen PM, Dijkstra PJ, Oberthur RC, Bantjes A, Feijen J, J. Agric. Food Chem., 48, 4326 (2000)

[18] Reichl S, Muller-Goymann CC, Eur. J. Pharm. Biopharm., 78, 432 (2011)

[19] Saul JM, Ellenburg MD, de Guzman RC, Dyke MV, J. Biomed. Mater. Res. A, 98, 544 (2011)

[20] Standards NCFCL, Performance Standards for Antimicrobial Disk Susceptibility Tests, National Committee for Clinical Laboratory Standards, 2003.

[21] Paswan S, Studying the Arsenic Absorption by Keratin Protein Extracted from Human Hair, Bachelor of Technology Thesis, National Institute of Technology Rourkela, 2012.

[22] Holzwarth G, Doty P, J. Am. Chem. Soc., 87, 218 (1965)

[23] Greenfield NJ, Nat. Protoc., 1, 2876 (2007)

[24] Yin XC, Li FY, He YF, Wang Y, Wang RM, Biomater. Sci., 1, 528 (2013)

[25] Ikkai F, Naito S, Biomacromolecules, 3(3), 482 (2002)