Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design

Yanling Quan; Lin Wang; Yisheng Liu; Jingxiang Cong; Shengquan Xie; Xiuhong Wu

Korean Chemical Engineering Research, Vol.53, No.3, 321-326, June, 2015

DOI10.9713/kcer.2015.53.3.321 Export Citation

Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design

Yanling Quan, Lin Wang, Yisheng Liu, Jingxiang Cong, Shengquan Xie¹, and Xiuhong Wu^†

Department of Chemistry and Engineering, University of Science and Technology of Liaoning, Anshan, Liaoning 114051, China
¹Liaoning Economic Vocational Technology Institute, Shenyang, Liaoning 110122, China

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Plackett-Burman (PB) and Box-Behnken (BB) experimental designs were used to optimize fermentation variables for the biotransformation of glycyrrhizin (GL) to monoglucuronyl-glycyrrhetinic acid (MGGA). The PB design was first used to screen the important factors among the medium variables. The steepest ascent method was used to approach the optimum range for each of these factors. The BB design was finally used to analyze the response surfaces of the screened factors for further optimization. The optimized conditions for this system were 0.7 g/L MgSO4·7H2O, 1.19 g/L GL, and cultivation for six days. The biotransformation of GL to MGGA could reach up to 35.72%, which is a good result for this kind of transformation.

Keywords:Glycyrrhizin;Aspergillus niger;Plackett-Burman Design;Biotransformation;Box-Behnken Design

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[Referenced By]

[1] Yun TK, Choi SY, Cancer. Epidem. Biomar., 4(4), 401 (1995)

[2] Rudakewich M, Ba F, Benishin CG, Planta. Med., 67, 533 (2001)

[3] Peng L, Sun S, Xie LH, Wicks SM, Xie JT, Cardiovasc. Ther., 30(4), 183 (2002)

[4] Wang ZX, Song JL, Yang GZ, Chin. J. Integr. Tradit. West. Med., 22, 796 (2002)

[5] Cong JX, Lin BC, J. Chromatogr. A, 1145, 190 (2007)

[6] Wang X, Zhang HI, Chin. Tradit. Herbal. Drugs., 36(11), 1741 (2005)

[7] Xia SH, Fang DC, Chin. Med. J., 120(10), 922 (2007)

[8] Clarke K, Lee FT, Brechbiel MW, Smyth FE, Old LJ, Scott AM, Clin. Cancer. Res., 6, 3621 (2000)

[9] Hou JG, Xue JJ, Wang CY, Liu L, Zhang DL, Wang Z, Li W, Zheng YN, Sung CK, World. J. Microbiol. Biotechnol., 28, 1807 (2012)

[10] Kim HS, Lee EH, Ko SR, Choi KJ, Park JH, Im DS, Arch. Pharm. Res., 27(4), 429 (2004)

[11] Sirikantaramas S, Asano T, Sudo H, Yamazaki M, Saito K, Curr. Pharm. Biotechno., 8, 196 (2007)

[12] Liu YC, Chen GY, Ge FL, Li W, Zeng LH, Cao WG, World. J. Microbiol. Biotechnol., 27, 759 (2011)

[13] Gouiric SC, Feresin GE, Tapia AA, Rossomando PC, Schmeda-Hirschmann G, Bustos DA, World. J. Microbiol. Biotechnol., 20, 281 (2004)

[14] Hu SC, Hong K, Song YC, Liu JY, Tan RX, World. J. Microbiol. Biotechnol., 25, 115 (2009)

[15] Zou SP, Zhou JJ, Kaleem I, Xie LP, Liu GY, Li C, Sep. Sci. Technol., 47(7), 1055 (2012)

[16] Li Y, Liu ZQ, Cui FJ, Liu ZH, Zhao H, Appl. Microbiol. Biotechnol., 77(2), 285 (2007)

[17] Palvannan T, Sathishkumar P, J. Basic. Microb., 50, 325 (2010)

[18] Plackett RL, Burman JP, Biometrika, 37, 305 (1946)

[19] Jacques P, Hbid C, Destain J, Razafindralambo H, Paquot M, De Pauw E, Thonart P, Appl. Biochem. Biotechnol., 77-79, 223 (1999)

[20] Yue PF, Zheng Q, Wu B, Hu PY, Wu ZF, Yang M, J. Dispersion Sci. Technol., 33, 213 (2012)

[21] Mahdavi V, Monajem A, Korean J. Chem. Eng., 33, 2178 (2013)

[22] Wu SJ, Yong ZJ, Zhu LH, Jin FX, Chinese. Tradit. Herbal. Drugs., 34, 516 (2003)