BMP-2 Immoblized in BCP-Chitosan-Hyaluronic Acid Hybrid Scaffold for Bone Tissue Engineering

Subrata Deb Nath; Celine Abueva; Swapan Kumar Sarkar; Byong Taek Lee

Korean Journal of Materials Research, Vol.24, No.12, 704-709, December, 2014

DOI10.3740/MRSK.2014.24.12.704 Export Citation

BMP-2 Immoblized in BCP-Chitosan-Hyaluronic Acid Hybrid Scaffold for Bone Tissue Engineering

Subrata Deb Nath¹, Celine Abueva¹, Swapan Kumar Sarkar², and Byong Taek Lee^{1, 2, †}

¹Department of Regenerative Medicine, Soonchunhyang University, Ssangyoung-dong, Cheonan, Chungnam 330-090, Korea
²Institute of Tissue Regeneration, Soonchunhyang University, Ssangyoung-dong, Cheonan, Chungnam 330-090, Korea

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In this study, we fabricated a novel micro porous hybrid scaffold of biphasic calcium phosphate (BCP) and a polylectrolyte complex (PEC) of chitosan (CS) and hyaluronic acid (HA). The fabrication process included loading of CSHA PEC in a bare BCP scaffold followed by lypophilization. SEM observation and porosimetry revealed that the scaffold was full of micro and macro pores with total porosity of more than 60 % and pore size in the range of 20~200 μm. The composite scaffold was mechanically stronger than the bare BCP scaffold and was significantly stronger than the CS-HA PEC polymer scaffold. Bone morphogenetic growth factor (BMP-2) was immobilized in CS-HA PEC in order to integrate the osteoinductive potentiality required for osteogenesis. The BCP frame, prepared by sponge replica, worked as a physical barrier that prolonged the BMP-2 release significantly. The preliminary biocompatibility data show improved biological performance of the BMP-2 immobilized hybrid scaffold in the presence of rabbit bone marrow stem cells (rBMSC).

Keywords:BCP;chitosan;hyaluronic acid;BMP-2;hybrid scaffold

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[2] Hollister SJ, Nat. Mater., 4(7), 518 (2005)

[3] Place ES, Evans ND, Stevens MM, Nat. Mater., 8(6), 457 (2009)

[4] Burg KJL, Porter S, Kellam JF, Biomaterials, 21(23), 2347 (2000)

[5] Olszta MJ, Cheng X, Jee SS, Kumar R, Kim YY, Kaufman MJ, Douglas EP, Gower LB, Mater. Sci. Eng. R-Rep., 58(3), 77 (2007)

[6] Deville S, Saiz E, Tomsia AP, Biomaterials, 27(32), 5480 (2006)

[7] Leong KF, Cheah CM, Chua CK, Biomaterials, 24(13), 2263 (2003)

[8] Hou Q, Grijpma DW, Feijen J, Biomaterials, 24(11), 1937 (2003)

[9] Vallet-Regi M, Gonzalez-Calbet JM, Prog. Solid State Chem., 32(1), 1 (2004)

[10] Roohani-Esfahani SI, Dunstan CR, Li JJ, Lu Z, Davies B, Pearce S, Field J, Williams R, Zreiqat H, Acta Biomaterials, 9(6), 7014 (2013)

[11] Masanori K, Koyama Y, Takakuda K, Miyairi H, Shirahama N, Tanaka J, J. Biomed. Mater. Res., 62(2), 265 (2002)

[12] Nath SD, Linh NTB, Sadiasa A, Lee BT, J. Biomater. App., 28(8), 1151 (2014)

[13] Phuong NT, Lee BT, J. Biomater. App., 27(3), 311 (2012)

[14] Francesco B, Ferraris M, Bretcanu O, Verne E, Vitale-Brovarone C, J. Biomater App., 1 (2011)

[15] Jayachandran V, Kim SK, Mar. Drugs, 8(8), 2252 (2010)

[16] Linh NTB, Lee BT, Tissue Engg Part A, (2014).

[17] Van Vlierberghe S, Dubruel P, Schacht E, Biomacromolecules, 12(5), 1387 (2011)

[18] Neffe AT, Wischke C, Racheva M, Lendlein A, Expert Rev. Med. Devices, 10(6), 813 (2013)

[19] Nath SD, Abueva C, Kim BR, Lee BT, Carbohydr. Polym. (2014)

[20] Kolambkar YK, Dupont KM, Boerckel JD, Huebsch N, Mooney DJ, Hutmacher DW, Guldberg RE, Biomaterials, 32(1), 65 (2011)

[21] Hannink G, Arts JJ, Injury, 42, S22 (2011)

[22] Bai F, Jinkang Z, Zhen W, Jianxi L, Jiang C, Jian L, Guolin M, Xin D, Biomedical Materials, 6(1), 015007 (2011)

[23] Murphy CM, Haugh MG, O'Brien FJ, Biomaterials, 31(3), 461 (2010)