초임계유체를 이용한 고삼으로부터 Genistein의 추출

한창남; 강춘형; Chang-Nam Han; Choon-Hyoung Kang

Korean Chemical Engineering Research, Vol.53, No.4, 445-449, August, 2015

DOI10.9713/kcer.2015.53.4.445 Export Citation

초임계유체를 이용한 고삼으로부터 Genistein의 추출

Extraction of Genistein from Sophora flavescens with Supercritical Carbon Dioxide

한창남, 강춘형 ^†

전남대학교 응용화학공학부, 500-757 광주시 북구 용봉동 300번지

Chang-Nam Han, and Choon-Hyoung Kang^†

Department of Applied Chemical Engineering, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Korea

E-mail:

초록

본 연구에서는 초임계 이산화탄소를 용매로 사용하여 고삼으로부터 genistein을 추출하였다. 공용매와 추출 온도 및 압력 등에 따른 추출효율을 측정하였으며, 공용매로 메탄올과 에탄올을 사용하여 200 bar에서 300 bar의 압력범위와 35 oC와 50 oC의 온도범위에서 연구를 수행하였다. 조건에 따라 서로 다른 genistein의 추출효율을 보였으며, 특히 높은 압력과 높은 온도에서 가장 높은 추출효율이 관찰되었다. 또한 메탄올이 에탄올보다 더욱 효과적인 공용매 특성을 보였으며 농도가 높을수록 추출효율도 증가하였다. DPPH 라디칼 소거 활성을 측정하여 고삼 추출물의 항산화 활성을 비교하였다.

This study was directed to finding an optimum extraction condition of genistein from the S. flavescens with supercritical carbon dioxide as a solvent. In this effort, effects of the extraction conditions including pressure, temperature and a co-solvent on the extraction efficiency were investigated. The aqueous ethanol and methanol solutions were used as co-solvents while the tested operating pressure and temperature ranges were from 200 bar to 300 bar and from 308.15 K to 323.15 K, respectively. The concentration of genistein was determined by means of HPLC equipped with a UV detector. From the results, it was observed that an increase in pressure led to the higher extraction efficiency. Further, methanol showed better performance as a co-slovent than ethanol. The DPPH radical scavenging activities were measured to compare antioxidant activities of S. flavescens extracts.

Keywords:Genistein;S. flavescens;Supercritical Extraction;Co-solvent;Radical Scavenging Activity

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[Referenced By]

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[1] Lee YW, News Inf. Chem. Eng., 19(3), 325 (2001)

[2] Lee YW, News Inf. Chem. Eng., 19(4), 457 (2001)

[3] Dobbs JM, Wong JM, Lahiere RJ, Johnston KP, Ind. Eng. Chem. Res., 26, 56 (1987)

[4] Lucien FP, Foster NR, J. Supercrit. Fluids, 17(2), 111 (2000)

[5] Mchugh MA, Krukonis VJ, Supercritical Fluid Extraction Principles and Practice, Butterworth-Heinemann, United States, US(1986).

[6] Shin MK, “Extract of Sophorae Radix for the Prevention and Treatment of Osteoporosis,” K. R. Patent No. 2002-0044746(2000).

[7] Kelly GE, “Treatment or Prevention of Menopausal Symptoms and Osteoporosis,” U.S. Patent No. 2002/0035074A1(2000).

[8] Wang G, Kuan SS, Francis OJ, Carman S, J. Agric. Food Chem., 38, 185 (1990)

[9] Peterson G, Barnes S, Biochem. Biophys. Res. Commun., 179, 661 (1991)

[10] Coward L, Barnes NC, Setchell KDR, Barnes S, J. Agric. Food Chem., 41, 661 (1961)

[11] Supko JG, Phillips LR, J. Chromatogr., 666, 157

[12] Levitzki A, Gazit A, Science, 267(5205), 1782 (1995)

[13] Jyong ME, Hwang IK, Korean Soybean Digest, 25(1), 23 (2008)

[14] So EH, Kuh JH, Park KY, Lee YH, Korean J. Bread, 33(1), 35 (2001)

[15] Record IR, Dreosti IE, Mclnerney JK, National Biochem., 6, 481 (1995)

[16] Yang ES, Hong RH, Kang SM, Korean J. Microbiol. Biotechnol., 35(4), 316 (2007)

[17] Oh HS, Kim JH, Lee MH, Korean J. Soc. Food Cookepy SCI., 19(3), 263 (2003)

[18] Barnes S, Blair HC, “Genistein for Use in Inhibiting Osteroclasts,” U.S. Patent No. 5, 506, 211(1996).

[19] Blois MS, Nature, 181, 1199 (1958)

[20] Myung JE, Hwang IK, Korean Soybean Digest, 25(1), 23 (2008)

[21] Jun HI, Denis P, Wiesenborn, Kim YS, Korean J. Food Preserv, 18(1), 59 (2011)

[22] Jeong GT, Lee KM, Park DH, Korean Chem. Eng. Res., 44(1), 81 (2006)