Integrated fabrication-conjugation approaches for biomolecular assembly and protein sensing with hybrid microparticle platforms and biofabrication - A focused minireview

Sukwon Jung; Hyunmin Yi

Korean Journal of Chemical Engineering, Vol.32, No.9, 1713-1719, September, 2015

DOI10.1007/s11814-015-0147-2 Export Citation

Integrated fabrication-conjugation approaches for biomolecular assembly and protein sensing with hybrid microparticle platforms and biofabrication - A focused minireview

Sukwon Jung, and Hyunmin Yi^†

Department of Chemical and Biological Engineering, Tufts University, 4 Colby St. Medford, MA 02155, U.S.A., USA

E-mail:

Controlled manufacturing of polymeric hydrogel microparticles is crucial, yet challenging, for rapid and sensitive detection of biomacromolecules in biodiagnostics and biosensing applications. Our approach is an integrated fabrication-conjugation strategy utilizing a simple and robust micromolding technique and biofabrication with a potent aminopolysaccharide chitosan as an efficient conjugation handle for high-yield bioorthogonal conjugation reactions. We present a concise overview of our recent findings in the controlled fabrication of shape-encoded or core-shell structured microparticles consisting of poly(ethylene glycol) (PEG) and short single-stranded (ss) DNA or chitosan, and their utility in the covalent conjugation and nucleic acid hybridization-based assembly of target ssDNAs, proteins and viral nanotemplates. Particularly, two novel routes to achieve substantially improved protein conjugation capacity and kinetics are presented from our recent reports: tobacco mosaic virus (TMV) as a high capacity nanotubular template and polymerization-induced phase separation (PIPS) of pre-polymer droplets for controlled core-shell structure formation. We envision that our fabrication-conjugation approaches reported here, combined with our current and future endeavors in improved fabrication and design of controlled structures with chemical functionalities, should permit a range of manufacturing strategies for advanced functional microscale materials and platforms in a wide array of applications.

Keywords:Micromolding;Hydrogel Microparticles;Biofabrication;Biomolecular Conjugation;Biosensing

[References]

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Uttamchandani M, Neo JL, Ong BNZ, Moochhala S, Trends Biotechnol., 27, 53 (2009)
Wulfkuhle JD, Liotta LA, Petricoin EF, Nat. Rev. Cancer, 3, 267 (2003)
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Pregibon DC, Doyle PS, Anal. Chem., 81, 4873 (2009)
Zubtsov DA, Savvateeva EN, Rubina AY, Pan’kov SV, Konovalova EV, Moiseeva OV, Chechetkin VR, Zasedatelev AS, Anal. Biochem., 368, 205 (2007)
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Park S, Lee HJ, Koh WG, Sensors-Basel, 12, 8426 (2012)
Ye BF, Zhao YJ, Li TT, Xie ZY, Gu ZZ, J. Mater. Chem., 21, 18659 (2011)
Ji XH, Zhang NG, Cheng W, Guo F, Liu W, Guo SS, He ZK, Zhao XZ, J. Mater. Chem., 21, 13380 (2011)
Zhao YJ, Shum HC, Chen HS, Adams LLA, Gu ZZ, Weitz DA, J. Am. Chem. Soc., 133(23), 8790 (2011)
Hu J, Zhao XW, Zhao YJ, Li J, Xu WY, Wen ZY, Xu N, Gu ZZ, J. Mater. Chem., 19, 5730 (2009)
Pregibon DC, Toner M, Doyle PS, Science, 315, 1393 (2007)
Appleyard DC, Chapin SC, Srinivas RL, Doyle PS, Nat. Protoc., 6, 1761 (2011)
Lewis CL, Choi CH, Lin Y, Lee CS, Yi H, Anal. Chem., 82, 5851 (2010)
Meagher RJ, Won JI, McCormick LC, Nedelcu S, Bertrand MM, Bertram JL, Drouin G, Barron AE, Slater GW, Electrophoresis, 26(2), 331 (2005)
Jung S, Yi H, Langmuir, 28(49), 17061 (2012)
Yi HM, Wu LQ, Bentley WE, Ghodssi R, Rubloff GW, Culver JN, Payne GF, Biomacromolecules, 6(6), 2881 (2005)
Koev ST, Dykstra PH, Luo X, Rubloff GW, Bentley WE, Payne GF, Ghodssi R, Lab Chip, 10, 3026 (2010)
Agnihotri SA, Mallikarjuna NN, Aminabhavi TM, J. Control. Release, 100, 5 (2004)
Croisier F, Jerome C, Eur. Polym. J., 49, 780 (2013)
Nelson DL, Lehninger AL, Cox MM, Lehninger Principles of Biochemistry, Freeman WH, New York (2008).
Jung S, Yi H, Biomacromolecules, 14(11), 3892 (2013)
Jung S, Yi HM, Langmuir, 30(26), 7762 (2014)
Choi CH, Jeong JM, Kang SM, Lee CS, Lee J, Adv. Mater., 24(37), 5078 (2012)
Jung S, Yi H, Chem. Mater., 27, 3988 (2015)
Yang CX, Choi CH, Lee CS, Yi HM, ACS Nano, 7, 5032 (2013)

[Referenced By]

[1] Baker KN, Rendall MH, Patel A, Boyd P, Hoare M, Freedman RB, James DC, Trends Biotechnol., 20, 149 (2002)

[2] Uttamchandani M, Neo JL, Ong BNZ, Moochhala S, Trends Biotechnol., 27, 53 (2009)

[3] Wulfkuhle JD, Liotta LA, Petricoin EF, Nat. Rev. Cancer, 3, 267 (2003)

[4] Fotin AV, Drobyshev AL, Proudnikov DY, Perov AN, MIrzabekov AD, Nucleic Acids Res., 26, 1515 (1998)

[5] Pregibon DC, Doyle PS, Anal. Chem., 81, 4873 (2009)

[6] Zubtsov DA, Savvateeva EN, Rubina AY, Pan’kov SV, Konovalova EV, Moiseeva OV, Chechetkin VR, Zasedatelev AS, Anal. Biochem., 368, 205 (2007)

[7] Meiring JE, Schmid MJ, Grayson SM, Rathsack BM, Johnson DM, Kirby R, Kannappan R, Manthiram K, Hsia B, Hogan ZL, Ellington AD, Pishko MV, Willson CG, Chem. Mater., 16, 5574 (2004)

[8] Park S, Lee HJ, Koh WG, Sensors-Basel, 12, 8426 (2012)

[9] Ye BF, Zhao YJ, Li TT, Xie ZY, Gu ZZ, J. Mater. Chem., 21, 18659 (2011)

[10] Ji XH, Zhang NG, Cheng W, Guo F, Liu W, Guo SS, He ZK, Zhao XZ, J. Mater. Chem., 21, 13380 (2011)

[11] Zhao YJ, Shum HC, Chen HS, Adams LLA, Gu ZZ, Weitz DA, J. Am. Chem. Soc., 133(23), 8790 (2011)

[12] Hu J, Zhao XW, Zhao YJ, Li J, Xu WY, Wen ZY, Xu N, Gu ZZ, J. Mater. Chem., 19, 5730 (2009)

[13] Pregibon DC, Toner M, Doyle PS, Science, 315, 1393 (2007)

[14] Appleyard DC, Chapin SC, Srinivas RL, Doyle PS, Nat. Protoc., 6, 1761 (2011)

[15] Lewis CL, Choi CH, Lin Y, Lee CS, Yi H, Anal. Chem., 82, 5851 (2010)

[16] Meagher RJ, Won JI, McCormick LC, Nedelcu S, Bertrand MM, Bertram JL, Drouin G, Barron AE, Slater GW, Electrophoresis, 26(2), 331 (2005)

[17] Jung S, Yi H, Langmuir, 28(49), 17061 (2012)

[18] Yi HM, Wu LQ, Bentley WE, Ghodssi R, Rubloff GW, Culver JN, Payne GF, Biomacromolecules, 6(6), 2881 (2005)

[19] Koev ST, Dykstra PH, Luo X, Rubloff GW, Bentley WE, Payne GF, Ghodssi R, Lab Chip, 10, 3026 (2010)

[20] Agnihotri SA, Mallikarjuna NN, Aminabhavi TM, J. Control. Release, 100, 5 (2004)

[21] Croisier F, Jerome C, Eur. Polym. J., 49, 780 (2013)

[22] Nelson DL, Lehninger AL, Cox MM, Lehninger Principles of Biochemistry, Freeman WH, New York (2008).

[23] Jung S, Yi H, Biomacromolecules, 14(11), 3892 (2013)

[24] Jung S, Yi HM, Langmuir, 30(26), 7762 (2014)

[25] Choi CH, Jeong JM, Kang SM, Lee CS, Lee J, Adv. Mater., 24(37), 5078 (2012)

[26] Jung S, Yi H, Chem. Mater., 27, 3988 (2015)

[27] Yang CX, Choi CH, Lee CS, Yi HM, ACS Nano, 7, 5032 (2013)