잣송이 부산물로부터 수증기 증류법에 의한 피톤치드의 추출

김배용; 이철태; Bae yong Kim; Chul-Tae Lee

Applied Chemistry for Engineering, Vol.26, No.6, 648-658, December, 2015

DOI10.14478/ace.2015.1064 Export Citation

잣송이 부산물로부터 수증기 증류법에 의한 피톤치드의 추출

Production of Phytoncide from Korean Pine Cone Waste by Steam Distillation

김배용, 이철태 ^†

단국대학교 화학공학과

Bae yong Kim, and Chul-Tae Lee^†

Department of Chemical Engineering, Dankook University, Gyeonggi 16890, Korea

E-mail:

초록

수증기 증류법을 이용하여 잣나무의 손상 없이 잣송이 부산물로부터 피톤치드 성분의 대량 추출 가능성을 조사하였다. 또한 수증기 증류법에 의해 얻어진 잣나무 피톤치드 oil을 대상으로 column chromatography법에 의한 피톤치드의 구성성분의 분리 가능성을 타진하였다. 잣송이 부산물로부터 물.수증기 증류법과 수증기 증류법에 의해 피톤치드의 대량생산이 가능하며, 수증기 증류법의 경우가 물-수증기 증류법의 경우에 비해 수율이 2배가량 높을 정도로 효과적이었다. 수증기 증류법의 경우 100 ℃, 30 min간 증류 시 잣나무 피톤치드 oil의 수율이 최고점이 얻어졌다. 얻어지는 잣나무 피톤치드 oil의 주성분은 α-pinene, β-pinene, D-limonene β-myrcene 및 3-carene 등 12가지 이상의 성분으로 이루어지고 있음을 확인하였다. 또한 수용성의 성분이 함유되어 있는 다량의 유효성분도 함께 생산되어지면 이들 성분은 oil상에서 확인된 물질과는 동일한 성분은 전혀 없는 verbenone, α-terpinieol, fenchol, 등의 성분으로 이루어짐을 확인하였다. 얻어지는 잣나무 피톤치드 oil의 다양한 구성 성분들은 column chromatography법에 의해 그 분리가 가능함을 확인하였다.

Extraction of phytoncide oil from korea pine cone waste without damaging the pine cone tree itself was investigated using a steam distillation method. Also various components in the extracted phytoncide oil were separated using a column chromatography method. The extraction of phytoncide oil was effectively proceeded, and the maximum production yield of phytoncide oil could be obtained under 100 ℃ of distillation temperature and within 30 minute of distillation time. According to chemical analysis, it was found that the phytoncide oil from korea pine cone waste was consisted of more than 12 components such as α-pinene, β-pinene, D-limonene, as main components. In addition, the aqueous hydrogel containing other components such as verbenone, α-terpinieol, fenchol, different from components of phytoncide oil itself could be obtained through the steam distillation.

Keywords:phytoncide;extraction;pine tree;pine cone waste;steam distillation

[References]

Yang JK, Kang BK, Korean J. Biotechnol. Bioeng., 17(4), 357 (2002)
Ahn JY, Lee SS, Kang HY, J. Korean Soc. Cosmet. Sci., 30(4), 503 (2004)
Ekyundyo O, J. Chromatogr. Sci., 16, 294 (1978)
Choi KS, Korean J. Food Sci. Technol., 20, 769 (1988)
Hong WT, Go GM, Lee JG, Jang HJ, Kwag JJ, J. Korean Soc. Tob. Sci., 24(1), 53 (2002)
Shim GS, Ahn WY, J. Kor. For. Energy, 9(2), 35 (1989)
Jang SJ, Kim YH, Kim MG, Kim GH, Yun SY, J. Korean Soc. Agric. Chem. Biotechnol., 45(2), 101 (2002)
Hwang BH, Jo JH, Ham SS, Kang HY, J. Korean Soc. Food Sci. Nutr., 29(1), 6 (2000)
Wen FJ, Study on the Analysis of Physicochemical Characteristics of Essential Oil and the Application to the Deodorants, PhD Dissertation, Kwangwoon University, Seoul, Korea (2006).
Jung HG, Bang JG, Sung NS, Kim SM, Korean J. Crop Sci., 48(S), 41 (2003)
Jo BS, The Screening of Biological Activity from Pinus koraiensis Siebold et Zucc. Leaf and Development to Functionality Product, MA Dissertation, Kyungpook National University, Sangju, Korea (2012).
Ok SJ, Hwang BH, J. Korean Soc. For. Energy Res., 10, 84 (1990)
Yu DY, A Study of Anti-oxidation Effect and Anti-bacterial Activation of Pinus koraiensis Extract, MA Dissertation, Kyonggi Unversity, Seoul, Korea (2010).
Song HJ, Metabolic Profiling of Terpenoids and its Antifungal and Insecticidal Activity of Thuja occidentalis L. MA Dissertation, Gyeongsang National University, Jinju, Korea (2011).
Kurita N, Miyaji M, Kurane R, Takahara Y, Agric. Biol. Chem., 45(4), 945 (1981)
Caccioni DRL, Guizzardi M, Biondi DM, Renda A, Ruberto G, Inter. J. Food Microbiol., 43, 73 (1998)
Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totte J, Pieters L, Vlietinck AJ, J. Ethnopharmacol., 79, 213 (2002)
Lee SS, Kang HY, Choi IG, Mokchae Konghak, 30(1), 48 (2002)
Morris JA, Khettry A, Seitz EW, J. Amer. Oil. Chem. Soc., 56, 595 (1979)

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[1] Yang JK, Kang BK, Korean J. Biotechnol. Bioeng., 17(4), 357 (2002)

[2] Ahn JY, Lee SS, Kang HY, J. Korean Soc. Cosmet. Sci., 30(4), 503 (2004)

[3] Ekyundyo O, J. Chromatogr. Sci., 16, 294 (1978)

[4] Choi KS, Korean J. Food Sci. Technol., 20, 769 (1988)

[5] Hong WT, Go GM, Lee JG, Jang HJ, Kwag JJ, J. Korean Soc. Tob. Sci., 24(1), 53 (2002)

[6] Shim GS, Ahn WY, J. Kor. For. Energy, 9(2), 35 (1989)

[7] Jang SJ, Kim YH, Kim MG, Kim GH, Yun SY, J. Korean Soc. Agric. Chem. Biotechnol., 45(2), 101 (2002)

[8] Hwang BH, Jo JH, Ham SS, Kang HY, J. Korean Soc. Food Sci. Nutr., 29(1), 6 (2000)

[9] Wen FJ, Study on the Analysis of Physicochemical Characteristics of Essential Oil and the Application to the Deodorants, PhD Dissertation, Kwangwoon University, Seoul, Korea (2006).

[10] Jung HG, Bang JG, Sung NS, Kim SM, Korean J. Crop Sci., 48(S), 41 (2003)

[11] Jo BS, The Screening of Biological Activity from Pinus koraiensis Siebold et Zucc. Leaf and Development to Functionality Product, MA Dissertation, Kyungpook National University, Sangju, Korea (2012).

[12] Ok SJ, Hwang BH, J. Korean Soc. For. Energy Res., 10, 84 (1990)

[13] Yu DY, A Study of Anti-oxidation Effect and Anti-bacterial Activation of Pinus koraiensis Extract, MA Dissertation, Kyonggi Unversity, Seoul, Korea (2010).

[14] Song HJ, Metabolic Profiling of Terpenoids and its Antifungal and Insecticidal Activity of Thuja occidentalis L. MA Dissertation, Gyeongsang National University, Jinju, Korea (2011).

[15] Kurita N, Miyaji M, Kurane R, Takahara Y, Agric. Biol. Chem., 45(4), 945 (1981)

[16] Caccioni DRL, Guizzardi M, Biondi DM, Renda A, Ruberto G, Inter. J. Food Microbiol., 43, 73 (1998)

[17] Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totte J, Pieters L, Vlietinck AJ, J. Ethnopharmacol., 79, 213 (2002)

[18] Lee SS, Kang HY, Choi IG, Mokchae Konghak, 30(1), 48 (2002)

[19] Morris JA, Khettry A, Seitz EW, J. Amer. Oil. Chem. Soc., 56, 595 (1979)