화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.32, 34-38, December, 2015
DOI10.1016/j.jiec.2015.09.016  Export Citation
Fabrication of Ag nanoaggregates/SiO2 yolk.shell nanoprobes for surface-enhanced Raman scattering
Taegyu Kang1, Sinyoung Jeong2, Homan Kang3, Jaehi Kim1, Hyung-Mo Kim4, San Kyeong1, Sang Hun Lee1, Dae Hong Jeong2, 3, Bong-Hyun Jun4, †, and Yoon-Sik Lee1, 3, †
  • 1School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea
  • 2Department of Chemistry Education, Seoul National University, Seoul 151-742, Republic of Korea
  • 3Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea
  • 4Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
E-mail:,
Surface-enhanced Raman scattering (SERS) has received much attention due to ultra-high sensitivity in detection of biological and chemical analytes. When SERS is used as tagging probe, several strong points such as high sensitivity and multiplexing capability can resolved several key issues with current optical tagging materials. Our group has reported synthesis of silica-coated silver nanoparticles (NPs) embedded silica NPs (SERS Dots) for bio-applications. Here, we designed and synthesized a new type of hollow-shell type SERS active NP (yolk.shell SERS Dots), which are aggregated silver NPs with a hollow silica nanostructure. The yolk.shell SERS Dots were prepared by adding NaBH4 to SERS Dots, resulting in the prepared NPs exhibiting 2.3 times stronger SERS signals due to aggregation of silver NPs inside the silica shells and generating several hot spots among those silver NPs. Moreover, biotin-conjugated yolk.shell type SERS dots successfully bound streptavidin-Cy5 to demonstrate the potential of yolk.shell SERS dots for bio-detection. These yolk.shell SERS Dots have great potential for bio-imaging and drug delivery applications.
Keywords:Surface-enhanced Raman scattering (SERS);Nanoparticle probe;Hollow silica nanoparticles;Yolk-shell type SERS dots;Bio-imaging
  1. Willets K, Van Duyne R, J. Phys. Chem., 58, 267 (2007)
  2. Nima ZA, Biswas A, Bayer IS, Hardcastle FD, Perry D, Ghosh A, Dervishi E, Biris AS, Drug Metab. Rev., 46, 155 (2014)
  3. Banholzer MJ, Millstone JE, Qin L, Mirkin CA, Chem. Soc. Rev., 37, 885 (2008)
  4. Wang YQ, Yan B, Chen LX, Chem. Rev., 113(3), 1391 (2013)
  5. Cao Y, Jin R, Mirkin C, Science, 297, 1536 (2002)
  6. Graham D, Mallinder B, Whitcombe D, Watson N, Smith W, Anal. Chem., 74, 1069 (2002)
  7. Palonpon AF, Ando J, Yamakoshi H, Dodo K, Sodeoka M, Kawata S, Fujita K, Nat. Protoc., 8, 677 (2013)
  8. Kircher MF, de la Zerda A, Jokerst JV, Zavaleta CL, Kempen PJ, Mittra E, Pitter K, Huang R, Campos C, Habte F, Sinclair R, Brennan CW, Mellinghoff IK, Holland EC, Gambhir SS, Nat. Med., 18, 829 (2012)
  9. Bosnick KA, Jiang J, Brus LE, J. Phys. Chem. B, 106(33), 8096 (2002)
  10. Lee SJ, Morrill AR, Moskovits M, J. Am. Chem. Soc., 128(7), 2200 (2006)
  11. Ru EL, Etchegoin P, Meyer M, J. Chem. Phys., 125, 204701 (2006)
  12. Kleinman SL, Frontiera RR, Henry AI, Dieringer JA, Van Duyne RP, Phys. Chem. Chem. Phys., 15, 21 (2013)
  13. Chen G, Wang Y, Yang MX, Xu J, Goh SJ, Pan M, Chen HY, J. Am. Chem. Soc., 132(11), 3644 (2010)
  14. Su X, Zhang J, Sun L, Koo T, Chan S, Sundararajan N, Yamakawa M, Berlin A, Nano Lett., 5, 49 (2005)
  15. Huang PJ, Chau LK, Yang TS, Tay LL, Lin TT, Adv. Funct. Mater., 19(2), 242 (2009)
  16. Lim DK, Jeon KS, Kim HM, Nam JM, Suh YD, Nat. Mater., 9(1), 60 (2010)
  17. He X, Yue C, Zang Y, Yin J, Sun S, Li J, Kang J, J. Mater. Chem. A, 1, 15010 (2013)
  18. Kim J, Kim J, Choi H, Lee S, Jun B, Yu K, Kuk E, Kim Y, Jeong D, Cho M, Anal. Chem., 78, 6967 (2006)
  19. Woo M, Lee S, Kim G, Baek J, Noh M, Kim J, Park S, Minai-Tehrani A, Park S, Seo Y, Anal. Chem., 81, 1008 (2009)
  20. Jun B, Noh M, Kim J, Kim G, Kang H, Kim M, Seo Y, Baek J, Kim J, Park J, Small, 6, 119 (2010)
  21. Kim HM, Kang YL, Chung WJ, Kyeong S, Jeong S, Kang H, Jeong C, Rho WY, Kim DH, Jeong DH, Lee YS, Jun BH, J. Ind. Eng. Chem., 21, 158 (2015)
  22. Kim JH, Kang H, Kim S, Jun BH, Kang T, Chae J, Jeong S, Kim J, Jeong DH, Lee YS, Chem. Commun., 47, 2306 (2011)
  23. Caruso F, Caruso RA, Mohwald H, Science, 282(5391), 1111 (1998)
  24. Sun Y, Xia Y, Science, 298, 2176 (2002)
  25. Xu XL, Asher SA, J. Am. Chem. Soc., 126(25), 7940 (2004)
  26. Zhu Y, Shi J, Shen W, Dong X, Feng J, Ruan M, Li Y, Angew. Chem.-Int. Edit., 44, 5083 (2005)
  27. Lou XW, Wang Y, Yuan CL, Lee JY, Archer LA, Adv. Mater., 18(17), 2325 (2006)
  28. Vadakkekara R, Chakraborty M, Parikh PA, J. Ind. Eng. Chem., 20(3), 767 (2014)
  29. Tan MN, Park YS, J. Ind. Eng. Chem., 15(3), 365 (2009)
  30. Kang H, Kang T, Kim S, Kim JH, Jun BH, Chae J, Park J, Jeong DH, Lee YS, J. Nanosci. Nanotechnol., 11, 579 (2011)
  31. Stober W, Fink A, Bohn E, J. Colloid Interface Sci., 26, 62 (1968)
  32. Lizmarzan LM, Giersig M, Mulvaney P, Langmuir, 12(18), 4329 (1996)
  33. Graf C, van Blaaderen A, Langmuir, 18(2), 524 (2002)
  34. Zhang T, Ge J, Hu Y, Zhang Q, Aloni S, Yin Y, Angew. Chem.-Int. Edit., 120, 5890 (2008)
  35. Zhang T, Zhang Q, Ge J, Goebl J, Sun M, Yan Y, Liu Y, Chang C, Guo J, Yin Y, J. Phys. Chem. C, 113, 3168 (2009)