화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.32, 99-108, December, 2015
DOI10.1016/j.jiec.2015.08.001  Export Citation
Simple adsorption of Candida rugosa lipase onto multi-walled carbon nanotubes for sustainable production of the flavor ester geranyl propionate
NurRoyhaila Mohamad, Nor Aziah Buang, Naji A. Mahat, Joazaizulfazli Jamalis, Fahrul Huyop1, Hassan Y. Aboul-Enein2, and Roswanira Abdul Wahab†
  • Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • 1Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • 2Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt
E-mail:
In this study, geranyl propionate was enzymatically synthesized from geraniol and propionic acid using Candida rugosa lipase immobilized on acid functionalized multi-walled carbon nanotubes. The efficiency of the CRL-MWCNTs biocatalysts to catalyze the esterification production of geranyl propionate (solvent log P, alcohol:acid molar ratio and thermal stability) was compared with the free CRL for parameters. The use of CRL-MWCNTs in n-heptane (log P 4.0) and alcohol:acid molar ratio of 5:1 resulted in a 2-fold increased conversion frequency as compared to the free CRL for the production of geranyl propionate, in addition to a noteworthy 2-fold enhanced thermal stability.
Keywords:Candida rugosa lipase;Immobilization;Multi-walled carbon nanotubes;Esterification;Geranyl propionate
  1. Radzi SM, Mustafa WAF, Othman SS, Noor HM, World Acad. Sci. Eng. Technol., 59, 677 (2011)
  2. Malcata FX, Reyes HR, Garcia HS, Hill CG, Amundson CH, J. Am. Oil Chem. Soc., 67, 890 (1990)
  3. Charpe TW, Rathod VK, Waste Manage., 31, 85 (2011)
  4. Paroul N, Grzegozeski LP, Ciaradia V, Treichel H, Cansian RL, Oliveira JV, de Olivera D, J. Chem. Technol. Biotechnol., 12, 1636 (2010)
  5. Solano DM, Hoyos P, Hernaiz MJ, Alcantara AR, Sanchez-Montero JM, Bioresour. Technol., 115, 196 (2012)
  6. Anastas P, Eghbali N, Chem. Soc. Rev., 39, 301 (2010)
  7. Nestl BM, Nebel BA, Hauer B, Curr. Opin. Chem. Biol., 15, 187 (2011)
  8. Tao J, Kazlaukas RJ, Biocatalysis for Green Chemistry and Chemical Process Development, John Wiley & Sons Ltd., Hoboken, New Jersey, 2011.
  9. Ben Salah R, Ghamghui H, Miled N, Mejdoub H, Gargouri Y, J. Biosci. Bioeng., 103(4), 368 (2007)
  10. Zhou G, Wu C, Jiang X, Ma J, Zhang H, Song H, Process Biochem., 47, 953 (2012)
  11. Wahab RA, Abdul Rahman MB, Basri M, Abdul Rahman RNZR, Salleh AB, Leow TC, Biotechnol. Biotechnol. Equip., 28, 1065 (2014)
  12. Kuang Y, Zhao L, Zhang S, Zhang F, Dong MD, Xu S, Materials, 3, 5220 (2010)
  13. Song J, Chen M, Regina VR, Wang C, Meyer RK, Xie E, Wang C, Besenbacher F, Dong M, Adv. Eng. Mater., 14, B240 (2012)
  14. Jaeger KE, Eggert T, Curr. Opin. Biotechnol., 13, 390 (2001)
  15. Shim M, Kam NWS, Chen RJ, Li YM, Dai HJ, Nano Lett., 22, 28 (2002)
  16. Zhang P, Henthorn DB, AIChE J., 56(6), 1610 (2010)
  17. Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R, Enzyme Microb. Technol., 40(6), 1451 (2007)
  18. Tischer W, Kascher V, Trends Biotechnol., 17, 326 (1999)
  19. Norouzian D, Iran. J. Biotechnol., 1, 197 (2003)
  20. de Oliveira PC, Alves GM, de Castro HF, Biochem. Eng. J., 5, 63 (2000)
  21. Bradford MM, Anal. Biochem., 72, 248 (1976)
  22. Langone MAP, Sant'Anna GL, Appl. Biochem. Biotechnol., 77-79, 759 (1999)
  23. Mohamad NR, Buang NA, Mahat NA, Lok YY, Huyop F, Aboul-Enein HY, Wahab RA, Enzyme Microb. Technol., 72, 49 (2015)
  24. Ramanathan T, Fisher FT, Ruoff RS, Brinson LC, J. Mater. Chem., 17, 1290 (2005)
  25. Prlainovic NZ, Bezbradica DI, Knezˇevic´-Jugovic ZD, Stevanovic SI, Ivic´ MLA, Uskokovic´ PS, Mijin DZ, J. Ind. Eng. Chem., 19(1), 279 (2013)
  26. Pavlidis IV, Tsoufis T, Enotiadis A, Gournis G, Stamatis H, Adv. Eng. Mater., 12, 5 (2010)
  27. Zhang L, Kiny VU, Peng H, Zhu JJ, Mater. Chem., 16, 2055 (2004)
  28. Alkhatib MF, Mirghani MES, Qudsieh IY, Husain IAF, J. Appl. Sci., 10, 2705 (2010)
  29. Natalello A, Ami D, Brocca S, Lotti M, Doglia SM, Biochem. J., 85, 511 (2005)
  30. Mohamad NR, Marzuki NHC, Buang NA, Huyop F, Wahab RA, Biotechnol. Biotechnol. Equip., 29, 205 (2015)
  31. Datsyuk V, Kalyva M, Papagelis K, Parthemios J, Tasis D, Carbon, 46, 833 (2008)
  32. Vicente JL, Albesa A, Llanos JL, Flores ES, Fertutta AE, J. Argent. Chem. Soc., 98, 29 (2011)
  33. Raghavendra T, Basak A, Manocha LM, Shah AR, Madamwar D, Bioresour. Technol., 140, 103 (2013)
  34. Castro HF, Silva MLCP, Silca GLJP, Braz. J. Chem. Eng., 17, 4 (2000)
  35. Shi Y, Jin F, Wu Y, Yan F, Yu X, Quan Y, Dalian Institute of Light Industry, 13, 111 (1997)
  36. Yudianti R, Onggo H, Sudirman R, Saito Y, Iwata T, Azuma JI, Open Mater. Sci. J., 5, 242 (2011)
  37. Alkhatib MF, Mirghani MES, Qudsieh IY, Husain IAF, J. Appl. Sci., 10(21), 2705 (2010)
  38. Johan MR, Suhaimy SHM, Yusof Y, Appl. Surf. Sci., 289, 450 (2014)
  39. Mazov I, Kuznetsov VL, Simonova IA, Stadnichenko AI, Ishchenko AV, Romanenko AI, Tkachev EN, Anikeeva OB, Appl. Surf. Sci., 258(17), 6272 (2012)
  40. Khani H, Moradi O, J. Nanostruct. Chem., 3 (2013)
  41. Gogoi S, Dutta NN, Indian J. Chem. Technol., 16(3), 209 (2009)
  42. Laane C, Boeren S, Vos K, Veeger C, Biotechnol. Bioeng., 30, 81 (1987)
  43. Song J, Kahveci D, Chen ML, Guo Z, Xie EQ, Xu XB, Besenbacher F, Dong MD, Langmuir, 28(14), 6157 (2012)
  44. Krishna SH, Karanth NG, Catal. Rev., 4, 499 (2002)
  45. Zaks A, Klibanov AM, Science, 22, 1949 (1984)
  46. Yang L, Dordick JS, Garde S, Biophys. J., 87, 812 (2004)
  47. Trani M, Ergan F, Andre G, J. Am. Oil Chem. Soc., 68, 20 (1991)
  48. Stamatis H, Christakopolous P, Kekos D, Macris BJ, Kolisis FN, J. Mol. Catal. B-Enzym., 4, 229 (1998)
  49. Cabral PP, Fonseca MMR, Dias SF, Biochem. Eng. J., 43, 327 (2009)
  50. Rahman MBA, Chaibakhsh N, Basri M, Rahman RNZRA, Salleh AB, Radzi SM, J. Chem. Technol. Biotechnol., 83(11), 1534 (2008)
  51. Dave R, Madamwar D, in: Larroche C, Pandey A, Dussap CG (Eds.), Current Topics on Bioprocesses in Food Industry, Asiatech Publishers Inc., New Delhi, 2006, p. 70.
  52. Wang L, Wei L, Chen Y, Jiang R, J. Biotechnol., 150, 57 (2010)
  53. Zhu K, Jutila A, Tuominen EKJ, Kinnunen PKJ, Protein Sci., 10, 339 (2001)
  54. Yilmaz E, Can K, Sezgin M, Yilmaz M, Bioresour. Technol., 102, 499 (2001)
  55. Thanighai RR, Nambikkairaj B, Sivamani P, Ind. J. Appl. Res., 3, 375 (2013)