화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.33, No.1, 120-125, January, 2016
DOI10.1007/s11814-015-0116-9  Export Citation
Fabrication of bioanode by using electrically conducting polythiophene via entrapment technique
Inamuddin†, Beenish, and Mu. Naushad1
  • Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India
  • 1Department of Chemistry, College of Science, King Saud University, Building #5, Riyadh-11451, Kingdom of Saudi Arabia
E-mail:
A glassy carbon electrode (GCE) was tailored with conducting polymer polythiophene and further immobilized by an enzyme glucose oxidase (GOx). A thin film of polymer was developed by electrochemical polymerization of thiophene monomer. During electrochemical polymerization of the monomer the enzyme GOx and the redox active mediator ferritin (Frt) were entrapped within this polymer matrix. In this novel approach, the entrapment of enzyme and mediators within a polymer matrix occurs without chemical reaction that could affect their activity. The entrapment of enzyme and mediator within the conducting polymer matrices increases the surface area of the electrode. The tailored GCE/Ptp/Frt/GOx electrode showed a high catalytic activity. The increased surface area causes a high rate of electron transfer between the electrode and Frt engaged as an electron transfer mediator. The electrochemical properties of the electrode were determined by cyclic voltammetry (CV) and linear sweep voltammetry (LSV). The fabricated bioanode showed a current density of 3.9mA cm.2 at 1.0 V vs. Ag/AgCl in a 45 mM glucose solution and suggests proficient chances in biofuel cells (BFCs) applications.
Keywords:Optimization;Conducting Polymer;Polythiophene;Entrapment;Glucose Oxidase
  1. Frank D, Seamus HPJ, Biosens. Bioelectron., 22, 1224 (2007)
  2. Bullen RA, Arnot TC, Lakeman JB, Walsh FC, Biosens. Bioelectron., 21, 2015 (2006)
  3. Katz E, Shipway A, Willner I, Handbook of fuel cells Fundamentals, Technology and Applications, Wiley, Jerusalem (2003).
  4. Shukla AK, Suraish P, Berchmans S, Rajendran A, Curr. Sci., 87, 455 (2004)
  5. ElMekawy A, Srikanth S, Vanbroekhoven K, De Wever H, Pant D, J. Power Sources, 262, 183 (2014)
  6. Ghasemi M, Ismail M, Kamarudin SK, Saeedfar K, Daud WRW, Hassan SHA, Heng LY, Alam J, Oh SE, Appl. Energy, 102, 1050 (2013)
  7. Kim Y, Logan BE, Desalination, 308, 115 (2013)
  8. Sevda S, Dominguez-Benetton X, Vanbroekhoven K, De Wever H, Sreekrishnan TR, Pant D, Appl. Energy, 105, 194 (2013)
  9. Rigla M, Hernando ME, Gomez EI, Brugues E, Garcia-Saez G, Capel I, Pons B, Deleiva A, Diabetes. Tech. Therapeut., 10, 194 (2008)
  10. Heller A, Annu. Rev. Biomed. Eng., 1, 193 (1999)
  11. Barton SC, Gallaway J, Atanassov P, Chem. Rev., 104(10), 4867 (2004)
  12. Heller A, Phys. Chem. Chem. Phys., 6, 209 (2004)
  13. Itamar W, Fuel Cells., 9, 1 (2009)
  14. Yahiro AT, Lee SM, Kimble DO, Biochim. Biophys. Acta, 88, 375 (1964)
  15. Zhang X, Pant D, Zhang F, liu J, He W, Logan BE, Chem. Electro. Chem., 1, 1859 (2014)
  16. Dominguez-Benetton X, Srikanth S, Satyawali Y, Vanbroekhoven K, Pant D, J. Microbial. Biochem. Technol. S6, 2, 20 (2013)
  17. Ramsay G, Wolper SM, Anal. Chem., 71, 504 (1999)
  18. Kojima K, Nasu H, Shimomura M, Miyauchi S, Synth. Met., 71, 2245 (1995)
  19. Shi L, Xiao Y, Willner I, Electrochem. Commun., 6, 1057 (2004)
  20. Kuwahara T, Oshima K, Shimomura M, Miyauchi S, Polymer, 46(19), 8091 (2005)
  21. Lee SU, Jung K, Park GW, Seo C, Hong YK, Hong WH, Chang HN, Korean J. Chem. Eng., 29(7), 831 (2012)
  22. Barriere F, Ferry Y, Rochefort D, Leech D, Electrochem. Commun., 6, 237 (2004)
  23. Soukharev V, Mano N, Heller A, J. Am. Chem. Soc., 126(27), 8368 (2004)
  24. Sato F, Togo M, Islam MK, Matsue T, Kosuge J, Fukasaru N, Kurosawa S, Nishizawa M, Electrochem. Commun., 7, 643 (2005)
  25. Hale PD, Inagaki T, Karan HI, Okamoto T, Skotheim TA, J. Am. Chem. Soc., 111, 3482 (1989)
  26. Nakano K, Doi K, Tamura K, Katsumi Y,Tazaki M, Chem. Commun., 13, 1544 (2003)
  27. Willner I, Helegshabtai V, Blonder R, Katz E, Tao GL, J. Am. Chem. Soc., 118(42), 10321 (1996)
  28. Xiao Y, Patolsky F, Katz E, Hainfeld JF, Willner I, Science, 299, 1877 (2003)
  29. Wang SC, Yang F, Silva M, Zarow A, Wang YB, Iqbal Z, Electrochem. Commun., 11, 34 (2009)
  30. Tan YM, Deng WF, Ge B, Xie QJ, Huang JH, Yao SZ, Biosens. Bioelectron., 24, 2225 (2009)
  31. Pingarron JM, Yanez-Sedeno P, Gonzalez-Cortes A, Electrochim. Acta, 53(19), 5848 (2008)
  32. Wong LS, Thirlway JL, Micklefield J, J. Am. Chem. Soc., 130, 12546 (2008)
  33. Bernfeld P, Wan J, Science, 142, 678 (1963)
  34. Brady D, Jordaan J, Biotechnol. Lett., 31(11), 1639 (2009)
  35. Jo SM, Xia Y, Lee HY, Kim YC, Kim JC, Korean J. Chem. Eng., 25(5), 1221 (2008)
  36. Bartlett PN, Cooper JM, J. Electroanal. Chem., 362(1-2), 1 (1993)
  37. Bartlett PN, Birkin PR, Synth. Met., 61, 15 (1993)
  38. Malhotra BD, Chaubey A, Singh SP, Anal. Chim. Acta, 578, 59 (2006)
  39. Ramanavicius A, Ramanaviciene A, Malinauskas A, Electrochim. Acta, 51(27), 6025 (2006)
  40. Kwon IC, Bae YH, Kim SW, Nature, 28, 291 (1991)
  41. Minteer SD, Liaw BYL, Cooney MJ, Curr. Opin. Biotechnol., 18, 228 (2007)
  42. Cosnier S, Shan D, Ding SN, Electrochem. Commun., 12, 266 (2010)
  43. Shin KM, Watt GD, Zhang B, Harb JN, Harrison RG, Kim SI, Kim SJ, J. Electroanal. Chem., 598(1-2), 22 (2006)
  44. Nazaruk E, Smolinski S, Swatko-Ossor M, Ginalska G, Fiedurek J, Rogalski J, Bilewicz R, J. Power Sources, 183(2), 533 (2008)
  45. Merle G, Habrioux A, Servat K, Rolland M, Innocent C, Kokoh KB, Tingry S, Electrochim. Acta, 54(11), 2998 (2009)
  46. Mano N, Mao F, Heller A, J. Electroanal. Chem., 574(2), 347 (2005)
  47. Singh V, Joung D, Zhai L, Das S, Khondaker SI, Seal S, Prog. Mater. Sci., 56(8), 1178 (2011)
  48. Agnes C, Reuillard B, Goff AL, Holzinger M, Cosnier S, Electrochem. Commun., 34, 105 (2013)
  49. Inamuddin, Shin KM, Kim SI, So I, Kim SJ, Electrochim. Acta, 54(16), 3979 (2009)
  50. Inamuddin, Ahmad K, Naushad M, Int. J. Hydrog. Energy, 39(14), 7417 (2014)
  51. Shin KM, Lee JW, Wallace GG, Kim SJ, Sens. Actuators B-Chem., 133, 393 (2008)