An integrated process for continuous cellulosic bioethanol production

Minhee Han; Yule Kim; Woo-Sung Cho; Gi-Wook Choi; Bong-Woo Chung

Korean Journal of Chemical Engineering, Vol.33, No.1, 223-229, January, 2016

DOI10.1007/s11814-015-0121-z Export Citation

An integrated process for continuous cellulosic bioethanol production

Minhee Han, Yule Kim, Woo-Sung Cho¹, Gi-Wook Choi^†, and Bong-Woo Chung^{1, †}

Changhae Advanced Institute of Technology, Changhae Ethanol Co., Ltd., Wonmnsung-ro, Dukjin-gu, Jeonju 561-203, Korea
¹School of Chemical Engineering, Chonbuk National University, Baekje-daero, Dukjin-gu, Jeonju 561-156, Korea

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A high-efficiency, integrated bioethanol production process was developed in this study, using Miscanthus as lignocellulosic biomass. The continuous process involved a twin-screw extruder, a pretreated biomass washing/dewatering process, and a saccharification/fermentation process. In addition, the integration process was designed for the reuse of pretreatment solution and the production of highly concentrated bioethanol. Pretreatment was performed with 0.72 M NaOH solution at 95 oC using an 80 rpm twin-screw speed and a flow rate of 90mL/min (18 g/min of raw biomass feeding). Following washing and dewatering steps, the pretreated biomass was subjected to simultaneous saccharification and bioethanol fermentation processes. The maximum ethanol concentration, yield from biomass, and total volume obtained were 59.3 g/L, 89.9%, and 60 L, respectively, using a pretreated biomass loading of 23.1% (w/v) and an enzyme dosage of 30 FPU/g cellulose. The results presented here constitute an important contribution toward the production of bioethanol from Miscanthus.

Keywords:Miscanthus;Twin-screw Extruder;Reuse of Pretreatment Solution;Integration Process;Simultaneous Saccharification and Fermentation (SSF)

[References]

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Ragauskas AJ, Nagy M, Kim DH, Eckert CA, Hallett JP, Liotta CL, Ind. Biotechnol., 2, 55 (2006)
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Lee SH, Teramoto Y, Endo T, Bioresour. Technol., 100(1), 275 (2009)
Lee SH, Inoue S, Teramoto Y, Endo T, Bioresour. Technol., 101(24), 9645 (2010)
Han M, Kang KE, Kim Y, Choi GW, Process Biochem., 48(3), 488 (2013)
Lamsal B, Yoo J, Brijwani K, Alavi S, Biomass Bioenerg., 34(12), 1703 (2010)
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Choi GW, Moon SK, Kang HW, Min J, Chung BW, J. Chem. Technol. Biotechnol., 84(4), 547 (2009)
Hui L, Kim N, Min J, Kang JW, Janog HN, Bioresour. Technol., 100, 3248 (2009)
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[2] Ragauskas AJ, Nagy M, Kim DH, Eckert CA, Hallett JP, Liotta CL, Ind. Biotechnol., 2, 55 (2006)

[3] Sorensen A, Teller PJ, Hilstrom T, Ahring BK, Bioresour. Technol., 99(14), 6602 (2008)

[4] de Vrije T, de Haas GG, Tan GB, Keijsers ERP, Claassen PAM, Int. J. Hydrog. Energy, 27(11-12), 1381 (2002)

[5] Eitzinger J, Kossler C, Theor. Appl. Climatol., 72, 245 (2002)

[6] Himken M, Lammet J, Neukirchen D, Czypionka-Kause U, Olfs HW, Plant Soil., 189, 117 (1997)

[7] Lewandowski I, Clifton-Brown JC, Andersson B, Brasch G, Chrsitian DG, Jorgensen U, Jones MB, Riche AB, Schwarz KU, Tayebi K, Teixeira F, Agron J., 95, 1274 (2003)

[8] Badger PC, Ethanol from cellulose: A general review in Trends in new crops and new uses (Ed.: Janick J, Whipkey A), ASHS Publishers, Alexandria VA (2002).

[9] Converse AO, Ooshima H, Burns DS, Appl. Biochem. Biotechnol., 24-25, 67 (1990)

[10] Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M, Bioresour. Technol., 96(6), 673 (2005)

[11] Patel SJ, Onkarappa R, Shobha KS, Electron. J. Environ. Agr. Food Chem., 6, 1921 (2007)

[12] Kumar P, Barrett DM, Delwiche MJ, Stroeve P, Ind. Eng. Chem. Res., 48(8), 3713 (2009)

[13] Diaz MJ, Cara C, Ruiz E, Romero I, Moya M, Castro E, Bioresour. Technol., 101(7), 2428 (2010)

[14] Hsu TA, Pretreatment of biomass in Handbook on bioethanol production and utilization (Applied Energy Technology Series) (Ed.: Wyman CE) Taylor & Francis, Washington DC (2010).

[15] Dale BE, Henk LL, Shiang M, Dev. Ind. Microb., 26, 223 (1984)

[16] Yoon HH, Korean J. Chem. Eng., 15(6), 631 (1998)

[17] Brownell HH, Yu EKC, Saddler JN, Biotechnol. Bioeng., 28, 792 (1986)

[18] Klinpratoom B, Ontanee A, Ruangviriyachai C, Korean J. Chem. Eng., 32(3), 413 (2015)

[19] Han M, Kim Y, Kim SW, Choi GW, J. Chem. Technol. Biotechnol., 86(12), 1527 (2011)

[20] Lee SH, Teramoto Y, Endo T, Bioresour. Technol., 100(1), 275 (2009)

[21] Lee SH, Inoue S, Teramoto Y, Endo T, Bioresour. Technol., 101(24), 9645 (2010)

[22] Han M, Kang KE, Kim Y, Choi GW, Process Biochem., 48(3), 488 (2013)

[23] Lamsal B, Yoo J, Brijwani K, Alavi S, Biomass Bioenerg., 34(12), 1703 (2010)

[24] Madson PW, Ethanol distillation: The fundamentals, in The alcohol Textbook, Ed. Ingledew WM, Kelsall DR, Austin GD, Kluspies C. Nottingham University Publishers, Nottingham, 292 (2009).

[25] Han M, Moon SE, Choi GW, Bioprocess Biosyst. Eng., 37(11), 2205 (2014)

[26] National Renewable Energy Laboratory, Standard Biomass Analytical Procedures, http://www.nrel.gov/biomass/analytical_procedures.html.

[27] Choi GW, Moon SK, Kang HW, Min J, Chung BW, J. Chem. Technol. Biotechnol., 84(4), 547 (2009)

[28] Hui L, Kim N, Min J, Kang JW, Janog HN, Bioresour. Technol., 100, 3248 (2009)

[29] Varga E, Klinke HB, Reczey K, Thomsen AB, Biotechnol. Bioeng., 88, 517 (2004)

[30] Mohagheghi A, Tucker M, Grohmann K, Wyman C, Appl. Biochem. Biotechnol., 33, 67 (1992)

[31] Han M, Kim Y, Koo B, Choi GW, Bioresources, 6(2), 1939 (2011)